We narrowed to 16,185 results for: grna

1. Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124287

   Purpose

   sgRNA against human Col7A1

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   Collagen type VII alpha 1 chain (COL7A1 Human)
   

   Use

   CRISPR

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

2. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)

   Plasmid

   #171102

   Purpose

   CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9 (Otx2 Mouse, S. pyogenes)
   

   Use

   CRISPR

   Promoter

   Ef1a

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

   Plasmid

   #121819

   Purpose

   pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126777

   Purpose

   Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   Sniper SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   F539S, M763I, K890N

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. NFATc3-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124288

   Purpose

   sgRNA against human NFATc3

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   NFATc3 nuclear factor of activated T cells (NFATC3 Human)
   

   Use

   CRISPR

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

6. Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124286

   Purpose

   sgRNA against human Col7A1

   Depositor

   Csaba Vizler, Katalin Josvay

   Insert

   Collagen type VII alpha 1 chain (COL7A1 Human)
   

   Use

   CRISPR

   Available Since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AgU6.695&MosU6.syn_3xP3-DsRed1-SV40 (Tandem 2_2 gRNAs)

   Plasmid

   #243013

   Purpose

   For cloning two gRNAs in tandem after BspQI digestion under An. gambiae U6.695 and U6.MosU6syn (synthetic mosquito U6) promoters.

   Depositor

   George Christophides

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Insect

   Available Since

   Sept. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCM100_ PB5'IR-hU6-gRNA-CS1- CAG-tdTomato- PB3'IR

   Plasmid

   #229995

   Purpose

   PiggyBac sgRNA cloning plasmid with tdTomato reporter with a capture sequence (cs1)

   Depositor

   Christian Mayer

   Article

   Dvoretskova et al Nat Neurosci. 2024 May;27(5):862-872. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   NotI site for NEBuilder HiFi DNA Assembly with ss…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-MYL2-inteinC-Sauri C aa439-1061-U6-Camk2d sgRNA7

   Plasmid

   #220129

   Purpose

   Expresses Sauri cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

   Insert

   MYL2, inteinC, nSauri Cas9C
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   MYL2

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-MYL2-inteinC-SpC aa714-1368-U6-Camk2d sgRNA7

    Plasmid

    #226916

    Purpose

    Expresses Sp cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

    Insert

    MYL2, inteinC, nSp Cas9C
    

    Use

    AAV

    Promoter

    MYL2

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBK1876-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

    Plasmid

    #223162

    Purpose

    Expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBK1861-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

    Plasmid

    #223161

    Purpose

    Expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH

    Plasmid

    #131683

    Purpose

    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1

    Depositor

    Christopher Richie

    Article

    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

    Inserts

    EGFP-KASH
    SpCas9 sgRNA vs mouse GRID1
    

    Use

    AAV

    Tags

    KASH

    Promoter

    EF1a and mU6

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CASI-InteinC-SpRY C aa714-1368-U6-Camk2d sgRNA

    Plasmid

    #209788

    Purpose

    Expresses SpRY cas9C by the constitutive CASI promoter and sgRNA targeting the oxidative activation sites of Camk2d by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al Circulation. 2024 May 28;149(22):1778-17

    Insert

    SpRY C, Camk2d sgRNA
    

    Use

    AAV

    Promoter

    CASI

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CASI-InteinC-SpG C aa714-1368-U6-Lmna sgRNA1

    Plasmid

    #208110

    Purpose

    Expresses SpG cas9C by the constitutive CASI promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

    Insert

    SpG C, U6, Lmna sgRNA1, scaffold
    

    Use

    AAV

    Promoter

    U6

    Available Since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCF827-sgNT-1_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Plasmid

    #211693

    Purpose

    U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and sgNT-1 guide RNA vector
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCF827-sgCIDE-1_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Plasmid

    #211696

    Purpose

    U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and sgCIDE-1 guide RNA vector
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCF827-sgNT-3_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Plasmid

    #211695

    Purpose

    U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and sgNT-3 guide RNA vector
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCF827-sgCIDE-3_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Plasmid

    #211698

    Purpose

    U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and sgCIDE-3 guide RNA vector
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCF827-sgCIDE-2_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Plasmid

    #211697

    Purpose

    U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and sgCIDE-2 guide RNA vector
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge