We narrowed to 6,593 results for: nls
-
Plasmid#163862Purposeheat shock-induced expression of Cas9 in C. elegansDepositorInsertCas9, C. elegans codon optimized with intron and SV40_NLS
UseCRISPRExpressionWormPromoterPhsp-16.48Available SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRL-GFPGO2(NGC)-PGK-Neo
Plasmid#136893PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPGO2(NGC)
UseLentiviralTags2xNLSMutationATG>ACGPromoterSFFVAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL-GFPGO2(NGT)-PGK-Neo
Plasmid#136892PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPGO2(NGT)
UseLentiviralTags2xNLSMutationATG>ACGPromoterSFFVAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_026
Plasmid#123838PurposeEncodes ha-tagged and nuclear localized Abscisic Acid Insensitive as a Type 4a part to be used in the MTK systemDepositorInsertHA::2xNLS::ABI
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_027
Plasmid#123839PurposeEncodes ha-tagged and nuclear localized Gibberellin Insensitive as a Type 4a part to be used in the MTK systemDepositorInsertHA::2xNLS::GAI
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_015
Plasmid#123678PurposeEncodes ConLS, chicken hypersensitivity site 4 insulator and locus of X-over P1 (loxP) as a Type 1 part to be used in the MTK systemDepositorInsertConnector LS-2xHS4-LoxP
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDA186
Plasmid#74250PurposeIntermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).DepositorInsertInducible peptide of the dPSTR
ExpressionBacterial and YeastPromoterpGPD1Available SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA171
Plasmid#74251PurposeIntermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).DepositorInsertInducible peptide of the dPSTR
TagsVenusExpressionBacterial and YeastPromoterpSTL1Available SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRISPRainbow Kit for Multiplex Labeling
Plasmid Kit#1000000079PurposePlasmids using dCas9 and gRNA scaffolds that bind fluorescent proteins to label multiple chromosomal loci.DepositorApplicationGenome EditingVector TypeLentiviral, Mammalian ExpressionEditing TypeCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-UTX-F
Plasmid#17438PurposeExpression of UTX in mammalian cellsDepositorAvailable SinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
MCP-M-MLV RT∆RNase H
Plasmid#213749PurposeFor circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cellsDepositorInsertMCP-M-MLV RT∆RNase H
UseCRISPRTagsBPNLSExpressionMammalianPromoterCMVAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-niCPE
Plasmid#213747PurposeFor circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cellsDepositorInsertMCP-nLbCas12a-M-MLV RT∆RNase H
UseCRISPRTagsBPNLSExpressionMammalianMutationD156R, R1138A in LbCas12aPromoterCMVAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9
Plasmid#84245PurposeExpresses direct fusion KRAB-Sp dCas9DepositorInsertsKRAB-tagBFP-Sp dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), HA tag, KRAB, and tagBFPExpressionMammalianPromoterCAG and PGKAvailable SinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
MMLV-RT(dRH) for Split-PE (pEH50)
Plasmid#190106PurposeExpresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE useDepositorInsertMMLV-RT(delta RNAseH)
TagsbpNLSExpressionMammalianMutationmutations from RT in PE2 and truncation of RNAse …PromoterCMVAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9
Plasmid#84247PurposeExpresses direct fusion VPR-Sp dCas9DepositorInsertsVPR-tagBFP-Sp dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), HA tag, VPR, and tagBFPExpressionMammalianPromoterCAG and PGKAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH-mono-MAD7
Plasmid#170134PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertMAD7(ErCas12a)
UseCRISPRExpressionPlantPromotermaize Ubiquitin-1, switchgrass Ubiquitin-1Available SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-nuCPE
Plasmid#213748PurposeFor circular RNA-mediated prime editor using LbCas12a-nuCPE in HEK293T cellsDepositorInsertMCP-LbCas12a-M-MLV RT∆RNase H
UseCRISPRTagsBPNLSExpressionMammalianMutationD156R in LbCas12aPromoterCMVAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA012
Plasmid#215974PurposeFragmid fragment: (N' terminus) Bipartite Nuclear Localization SignalDepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCH68 (dAsCas12a-3xKRAB piggyBac)
Plasmid#217334PurposepiggyBac expression of dAsCas12a-3xKRABDepositorInsertdAsCas12a
UseCRISPRTagsnucleoNLS-3xKRAB-3xHA-P2A-TagBFP2ExpressionMammalianMutationE993APromoterCAGAvailable SinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYN2_1
Plasmid#184757PurposePlasmid for genome editing by CRISPR/Cas9DepositorInsertsCas9
sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
UseSynthetic BiologyTags2xNLSExpressionBacterial and YeastPromoterPGK1 and tRNA-Phe-HDV RibozymeAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only