We narrowed to 10,308 results for: nls

1. pGL4.23-CD69 CaRE3 (plus strand)

   Plasmid

   #91844

   Purpose

   Luciferase reporter for CD69 enhancer (IGI-P0621)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   CD69 CaRE3 (plus strand) (CD69 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Missing 1 base from 13 base polyT tract beginning…

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.23-IL2RA CaRE5 (plus strand)

   Plasmid

   #91841

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0618)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE5 (plus strand) (IL2RA Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Perfect match to reference sequence

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.23-IL2RA CaRE3 (minus strand)

   Plasmid

   #91835

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0612)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE3 (minus strand) (IL2RA Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   rs7893324 A>G, rs7090530 G>T, rs7090512 A&g…

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.23-IL2RA CaRE4 SNP (plus strand)

   Plasmid

   #91851

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0346)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE4 SNP (plus strand) (IL2RA Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   rs61839660 C>T

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL4.23-IL2RA CaRE5 (minus strand)

   Plasmid

   #91836

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0613)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE5 (minus strand) (IL2RA Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Perfect match to reference sequence

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL4.23-IL2RA CaRE2 (plus strand)

   Plasmid

   #91846

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0623)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE2 (plus strand) (IL2RA Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   rs6602425 T>G, T>C at chr10:6117603

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4.23-IL2RA CaRE2 (minus strand)

   Plasmid

   #91847

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0624)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE2 (minus strand) (IL2RA Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   rs6602425 A>C, G>A mutation at chr10:611706…

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4.23-IL2RA CaRE6 (minus strand)

   Plasmid

   #91840

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0617)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE6 (minus strand) (IL2RA Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   rs7893467 C>A

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.23-IL2RA CaRE6 (plus strand)

   Plasmid

   #91839

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0616)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE6 (plus strand) (IL2RA Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   rs7893467 G>T, rs11256448 A>G

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4.23-IL2RA CaRE1 (minus strand)

    Plasmid

    #91838

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0615)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE1 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. CRE recombinase

    Plasmid

    #62730

    Purpose

    Expresses Cre recombinase protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    Cre recombinase
    

    Use

    Cre/Lox

    Tags

    His tag and NLS

    Expression

    Bacterial

    Mutation

    Codon optimazed for e.coli

    Promoter

    T7

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. dead Sa Cas9

    Plasmid

    #138162

    Purpose

    For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    dSaCas9
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    D10A + N580A

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGM47523

    Plasmid

    #153221

    Purpose

    Level 0 zCas9i vector

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    SpCas9 with introns and 2 NLS
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFC332

    Plasmid

    #87845

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    hph (hygromycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ150

    Plasmid

    #69301

    Purpose

    Gateway entry vector with pcoCas9 (Plant codon optimized)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pcoCas9 (plant codon-optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Mutation

    NLS fusion

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFC333

    Plasmid

    #87844

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    ble (bleomycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMAL-his-LbCpf1-EC

    Plasmid

    #79008

    Purpose

    Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    LbCpf1
    

    Tags

    MBP-6xHis-NLS

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFC330

    Plasmid

    #87842

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    pyrG
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-CibN-dCas9-CibN

    Plasmid

    #60553

    Purpose

    Expresses CibN-dCas9-CibN in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    dCas9-CibN
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. PABE-7

    Plasmid

    #115628

    Purpose

    Targeted A to G in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-nCas9-3*NLS
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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