We narrowed to 9,042 results for: nls
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Plasmid#113411PurposeExpresses hA3B-BE3 in mammalian cellsDepositorInserthA3B-BE3 (APOBEC3B Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceOct. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGfa2-nLac
Plasmid#53126PurposeExpression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.DepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutationcontains -2163 to +47 (the human gfa2 segment), w…Promoterhuman GFAPAvailable SinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTE4398
Plasmid#74042PurposeExpresses human codon-optimized LbCpf1 and Lb crRNA.DepositorInsertsLb crRNA
LbCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMV and human U6Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
MO91-dCas9-AID(P182X)
Plasmid#83260PurposeExpresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.DepositorInsertdCas9-AID P182X
UseRetroviralTags3xFLAG-NLS and FLAGExpressionMammalianMutationD10A and H840A in Cas9; P182X in AIDPromoterMSCVAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTDH3-dCas9
Plasmid#46920PurposeYeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoterDepositorInsertdCas9
UseCRISPRExpressionYeastPromoterTDH3Available SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTE4396
Plasmid#74041PurposeExpresses human codon-optimized AsCpf1 and As crRNA.DepositorInsertsAs crRNA
AsCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMV and human U6Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
SID4x-dCas9-KRAB
Plasmid#106399PurposeExpresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domainDepositorAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pY011 (pcDNA3.1-hLb2Cpf1)
Plasmid#69983PurposeExpresses humanized Lb2Cpf1DepositorInserthLb2Cpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-OC-IRES-BSD
Plasmid#53118PurposeTo create stable cell clones with high-level expression of Cas9 and OCT1DepositorInsertsUseLentiviralTagsIRES-BSD, NLS, and P2AExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable SinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUDC175
Plasmid#103019PurposeS. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoterDepositorInsertFncpf1
Tags3HA and NLSExpressionYeastMutationhuman codon optimizedPromoterTEF1Available SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-deSpCas9
Plasmid#92114PurposeExpression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)DepositorInsertdead/inactive eSpCas9 with FLAG tag
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationD10A, H840A, K848A, K1003A, R1060APromoterCbhAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3D-BE3
Plasmid#113413PurposeExpresses hA3D-BE3 in mammalian cellsDepositorInserthA3D-BE3 (APOBEC3D Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAc-y1sgRNA-Cas9
Plasmid#49331PurposeExpresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cellsDepositorInsertsUseCRISPRTags3xFLAG and NLSExpressionInsectMutationHuman codon optimisedPromoterActin-5c and Drosophila U6Available SinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pY018 (pcDNA3.1-hPdCpf1)
Plasmid#69990PurposeExpresses humanized PdCpf1DepositorInserthPdCpf1
UseCRISPRTagsNLS & 3xHAExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3C-BE3
Plasmid#113412PurposeExpresses hA3C-BE3 in mammalian cellsDepositorInserthA3C-BE3 (APOBEC3C Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3F-BE3
Plasmid#113414PurposeExpresses hA3F-BE3 in mammalian cellsDepositorInserthA3F-BE3 (APOBEC3F Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pY012 (pcDNA3.1-hCMtCpf1)
Plasmid#69984PurposeExpresses humanized CMtCpf1DepositorInserthCMtCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-dSpCas9-HF1
Plasmid#92115PurposeExpression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)DepositorInsertdead/inactive SpCas9-HF1 with FLAG tag
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationD10A, N497A, R661A, Q695A, H840A, Q926APromoterCbhAvailable SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY017 (pcDNA3.1-hPcCpf1)
Plasmid#69989PurposeExpresses humanized PcCpf1DepositorInserthPcCpf1
UseCRISPRTagsNLS & 3xHAExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTE4254
Plasmid#89050PurposeExpresses St chimeric gRNA and human codon-optimized StCas9DepositorInsertsSt chimeric gRNA
hStCas9
UseCRISPRTagsSV40 NLSExpressionMammalianPromoterCMV and human U6Available SinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only