Paired CRISPR screens identify mitochondrial metabolism and UBE2H as aneuploid-specific dependencies in human cancer cell lines
Klaske M. Schukken, Saron M. Akalu, Charles Zou, Pranav K. Kandikuppa, Ryan A. Hagenson, Jessica L. Keane, Mason P. Lynch, Toyoki Yoshimoto, Olaf Klingbeil, Erin L. Sausville, Sanat Mishra, Christopher R. Vakoc, Zuzana Storchova, Sarah J. Aitken, Jason M. Sheltzer
bioRxiv 2026.04.26.720636
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254529 | pLenti_Cas9_GFP_AAVS1_sg1 | AAVS1 knockout, control guide |
| 254530 | pLenti_Cas9_GFP_AAVS1_sg2 | AAVS1 knockout, control guide |
| 254531 | pLenti_Cas9_GFP_AAVS1_sg3 | AAVS1 knockout, control guide |
| 254532 | pLenti_Cas9_GFP_ROSA26_sg1 | ROSA26 control guide |
| 254533 | pLenti_Cas9_GFP_CDC7_sg1 | CDC7 knockout |
| 254534 | pLenti_Cas9_GFP_CDC7_sg2 | CDC7 knockout |
| 254535 | pLenti_Cas9_GFP_CDC7_sg3 | CDC7 knockout |
| 254536 | pLenti_Cas9_GFP_CDC7_sg4 | CDC7 knockout |
| 254537 | pLenti_Cas9_GFP_CDK9_sg1 | CDK9 knockout |
| 254538 | pLenti_Cas9_GFP_CDK9_sg2 | CDK9 knockout |
| 254539 | pLenti_Cas9_GFP_CDK9_sg3 | CDK9 knockout |
| 254540 | pLenti_Cas9_GFP_CDK9_sg4 | CDK9 knockout |
| 254544 | pLenti_Cas9_GFP_UBE2H_sg4 | UBE2H knockout |
| 254545 | pLenti_Cas9_mCherry_UBE2H_sg4 | UBE2H knockout |