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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,011 results for: grn

Showing: 6481 - 6500 of 16011 results

  1. pGG369

    Plasmid
    #165606
    Purpose
    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…
    Expression
    Bacterial
    Mutation
    Recoding of BsaI site in the AmpR cassette, two B…
    Promoter
    lacUV2 driving ω-dCas9 and UV5 driving sgRNA
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165606-plasmid-map-sequence-id-330964

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  2. pUC-H1-Esp3I-mU6-Kan

    Plasmid
    #213015
    Purpose
    Vector for Cloning of multiple gRNAs driven by distinct promoters (H1 and mU6)
    Depositor
    Stephanie Roessler
    Insert
    H1 promoter, mU6 promoter, gRNA scaffolds
    Use
    Tags
    Expression
    Mutation
    Promoter
    H1 promoter
    Available Since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213015-plasmid-map-sequence-id-423154

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  3. pFG018

    Plasmid
    #62817
    Purpose
    Bacterial inducible gRNA expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available Since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    62817-plasmid-map-sequence-id-114422

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  4. pROS15_MATz

    Plasmid
    #166100
    Purpose
    Expression of dual gRNA targeting the MAT locus
    Depositor
    Rebecca Burgess
    Insert
    MAT Z1
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166100-plasmid-map-sequence-id-328822

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  5. pFG001

    Plasmid
    #62816
    Purpose
    Bacterial constitutive gRNA expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PN25
    Available Since
    Nov. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    62816-plasmid-map-sequence-id-123824

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  6. pMZ379

    Plasmid
    #74215
    Purpose
    Combined sgRNA/Cas9 vector with NatMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available Since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74215-plasmid-map-sequence-id-139305

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  7. pMZ377

    Plasmid
    #74214
    Purpose
    Combined sgRNA/Cas9 vector with LEU2 marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available Since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74214-plasmid-map-sequence-id-141045

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  8. pMZ376

    Plasmid
    #74213
    Purpose
    Combined sgRNA/Cas9 vector with KanMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available Since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74213-plasmid-map-sequence-id-139302

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  9. pUC-tRNAGln-BbsI-sU6-Kan

    Plasmid
    #213016
    Purpose
    Vector for Cloning of multiple gRNAs driven by distinct promoters (tRNA-Gln and synthetic U6)
    Depositor
    Stephanie Roessler
    Insert
    tRNAGln promoter, sU6 promoter, gRNA scaffolds
    Use
    Tags
    Expression
    Mutation
    Promoter
    tRNAGln promoter
    Available Since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213016-plasmid-map-sequence-id-423155

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  10. LentiGuide-10X-CS1-3'-puro

    Plasmid
    #212011
    Purpose
    Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)
    Depositor
    Danwei Huangfu
    Insert
    gRNA scaffold
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    212011-plasmid-map-sequence-id-419682

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  11. pK7-g7g8-K8

    Plasmid
    #218220
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218220-plasmid-map-sequence-id-432226

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  12. pK3-g3g4-K4

    Plasmid
    #218218
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    May 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    218218-plasmid-map-sequence-id-432228

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  13. pK5-g5g6-K6

    Plasmid
    #218219
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    May 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    218219-plasmid-map-sequence-id-432227

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  14. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid
    #214690
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214690-plasmid-map-sequence-id-428775

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  15. pDual_dsCas9_Blast_hs_TP73

    Plasmid
    #214691
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214691-plasmid-map-sequence-id-428772

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  16. pHnS KI;Pdha1 Donor;3xV5 KO;Mff

    Plasmid
    #240301
    Purpose
    KI:Pdha1 Donor:3xV5 KO:MFF
    Depositor
    Yuki Ogawa
    Insert
    KI gRNA for Pdha1
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    240301-plasmid-map-sequence-id-478529

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  17. pHnS KI;Lmnb1 Donor;3xV5 KO;Mecp2

    Plasmid
    #240298
    Purpose
    KI:Lmnb1 Donor:3xV5 KO:Mecp2
    Depositor
    Yuki Ogawa
    Insert
    KI gRNA for Lmnnb1
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    240298-plasmid-map-sequence-id-478524

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  18. pHnS KI;Gria1 Donor;3xV5 KO;Dlg4

    Plasmid
    #240294
    Purpose
    KI:Gria1 Donor:3xV5 KO:Dlg4
    Depositor
    Yuki Ogawa
    Insert
    KI gRNA for Gria1
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    240294-plasmid-map-sequence-id-478522

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  19. pHnS KI;Lmnb1 Donor;3xV5 KO;Rbfox3

    Plasmid
    #240299
    Purpose
    KI:Lmnb1 Donor:3xV5 KO:NeuN
    Depositor
    Yuki Ogawa
    Insert
    KI gRNA for Lmnnb1
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    240299-plasmid-map-sequence-id-478518

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  20. pHnS KI;Sptbn4 Donor;3xV5 KO;Nfasc

    Plasmid
    #240296
    Purpose
    KI:Sptbn4 Donor:3xV5 KO:Nfasc
    Depositor
    Yuki Ogawa
    Insert
    KI gRNA for Sptbn4
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available Since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240296-plasmid-map-sequence-id-478298

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Showing: 6481 - 6500 of 16011 results