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pPBpuro-UbC-canisTgfa

Plasmid

#173847

Purpose

Encoding dog Tgfa.

Depositor

Michiyuki Matsuda

Publication

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Tgfa.

Expression

Mammalian

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisEreg

Plasmid

#173848

Purpose

Encoding dog Ereg.

Depositor

Michiyuki Matsuda

Publication

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Tgfa.

Expression

Mammalian

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-neo-dTgfa

Plasmid

#173842

Purpose

A knockout vector for the dog Tgfa.

Depositor

Michiyuki Matsuda

Publication

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTEF1-UDH

Plasmid

#166679

Purpose

Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).

Depositor

Claudia Vickers

Publication

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Insert

UDH

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_CBX1_Chromo-shadow

Plasmid

#109965

Purpose

Protein expression and purification of CBX1_Chromo-shadow

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CBX1_Chromo-shadow (CBX1 Human)

Expression

Bacterial

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_IGLL1_C1-set

Plasmid

#109991

Purpose

Protein expression and purification of IGLL1_C1-set

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

IGLL1_C1-set (IGLL1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_KARS_tRNA-anti-codon

Plasmid

#109865

Purpose

Protein expression and purification of KARS_tRNA-anti-codon

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

KARS_tRNA-anti-codon (KARS1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-OMACS

Plasmid

#136765

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Acsm4

Depositor

Peter Mombaerts

Publication

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Acsm4 (Acsm4 Mouse)

Use

In situ probe

Mutation

ISH Probe insert, A102T- please see depositor com…

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140170

Purpose

Expresses a regulatable secretory cargo for Arabidopsis thaliana

Depositor

Benjamin Glick

Publication

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Expression

Plant

Promoter

CaMV 35S

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pUAS-attB V5 dDispatched delta CS HA

Plasmid

#126412

Purpose

Expression of V5 dDispatched Delta CS HA in Drosophila cells lines

Depositor

Stacey Ogden

Publication

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

d-Dispatched1 (disp Fly)

Tags

HA

Expression

Insect

Mutation

Disp1 Cleavage site

Available since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-VRER-P2A-Puro

Plasmid

#110849

Purpose

Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRER

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-P2A-Puro

Plasmid

#110850

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-VRER-PGK-Puro

Plasmid

#110856

Purpose

Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VRER

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pME GFP-DCX

Plasmid

#105962

Purpose

entry clone for gateway cloning

Depositor

Caren Norden

Publication

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

NP_835365.1 (DCX Human)

Tags

6xHis-thrombin cleavage site and EGFP

Expression

Bacterial

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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GST-AR-1-36

Plasmid

#104192

Purpose

Expresses human AR aa 1-36 in pGEX-5x-1 bact expression vector with GST

Depositor

Elizabeth Wilson

Publication

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

human androgen receptor aa 1-36 (AR Human)

Tags

GST

Expression

Bacterial

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-MAP4-C-10

Plasmid

#57794

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

MAP4

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
miniSOG-MAP4-C-10

Plasmid

#57772

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

MAP4

Tags

miniSOG

Expression

Mammalian

Mutation

G982V and G993C compared to NM_008633.4.

Promoter

CMV

Available since

Dec. 7, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PA-mCherry-miniSOG-CAF1-C-10

Plasmid

#57784

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

CAF1

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
PA-mCherry-miniSOG-C-Src-7

Plasmid

#57785

Purpose

Localization: Membrane/Vesicles, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

C-Src

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-Talin-N-10

Plasmid

#55096

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Talin

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only