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pCDFDuet-1:ChsH

Plasmid

#257762

Purpose

For expression of untagged ChsH

Depositor

Wilfred van der Donk

Article

Chen et al Proc Natl Acad Sci U S A. 2026 Apr 7;123

Insert

ChsH

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1:HymH

Plasmid

#257763

Purpose

For expression of untagged HymH

Depositor

Wilfred van der Donk

Article

Chen et al Proc Natl Acad Sci U S A. 2026 Apr 7;123

Insert

HymH

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pTarget_dnaA-PAmCherry2.1

Plasmid

#212605

Purpose

Contains the E. coli codon-optimised gene of the photoactivatable fluorescent protein PAmCherry2(M10L), together with a sgRNA for SpydCas9 targeting the E. coli dnaA gene.

Depositor

John van der Oost

Article

Olivi et al Nat Commun. 2025 Aug 21;16(1):7813. doi:

Insert

PAmCherry2.1

Use

CRISPR

Mutation

M10L

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-GADT7

Plasmid

#248657

Purpose

Empty vector for generating yeast 2-hybrid GAL4 activation domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.

Depositor

Sarah Assmann, John Doonan

Article

Rossignol et al J Cell Sci. 2007 Oct 15;120(Pt 20):3678-

Type

Empty backbone

Use

Yeast 2-hybrid

Tags

GAL4 activation domain, HA

Expression

Yeast

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pET-21(+)-His-zf438-NB23

Plasmid

#247372

Purpose

Express zf438-NB23 that bind to SARS-CoV-19 Spike protein cooperatively with NB15-T7 RNAP.

Depositor

Sebastian Maerkl

Article

Lee et al bioRxiv 2025.06.18.660355

Insert

His-zf438-NB23

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5429

Plasmid

#229036

Purpose

Expression vector for EilR: PT7-lacO-B0035-EilR-T7 terminator, AmpR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-RBS35-EilR-T7term

Tags

8x His-tag

Expression

Bacterial

Promoter

PT7-lacO

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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LLPS-pCDH-DDX6-CT

Plasmid

#240014

Purpose

Expresses human DDX6-CT fused to EGFP in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6-CT (303-483)

Use

Lentiviral

Tags

6xHis and EGFP

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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lenti-hygro-Mouse-sgDdx6-1

Plasmid

#240003

Purpose

Expresses Mouse sgDdx6 in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

sgDdx6

Use

Lentiviral

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMB22

Plasmid

#223006

Purpose

Control plasmid without Cas and Csy4 for Lotus callus assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Not applicable, control plasmid

Use

CRISPR

Expression

Plant

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMB24

Plasmid

#223008

Purpose

Control plasmid without Cas and Csy4 for Nicotiana leaf assay

Depositor

Martin Parniske

Article

Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

Insert

Not applicable, control plasmid

Use

CRISPR

Expression

Plant

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb12567

Plasmid

#219927

Purpose

PrCYC with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter CYC

Expression

Yeast

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb12566

Plasmid

#219926

Purpose

PrDGA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter DGA

Expression

Yeast

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLPC53

Plasmid

#209962

Purpose

Contains Level 0 Part: 5' Connector (5C1CSN with double terminator Tcat+sai) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

5' Connector (5C1CSN_Tcat+sai)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBP-P_nahR-PSalTTC

Plasmid

#204074

Purpose

Type 0 promoter part

Depositor

Mikhail Shapiro

Insert

nahR repressor and PSalTTC sodium salicylate-inducible promoter

Expression

Bacterial

Mutation

None

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12622

Plasmid

#219929

Purpose

PrACT with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter ACT

Expression

Yeast

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12562

Plasmid

#219923

Purpose

PrGPD with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter GPD

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13186

Plasmid

#219924

Purpose

PrTEF with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter TEF

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12561

Plasmid

#219922

Purpose

PrPGM with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter PGM

Expression

Yeast

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12565

Plasmid

#219925

Purpose

PrFBA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter FBA

Expression

Yeast

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGM295

Plasmid

#220183

Purpose

Expresses exogenous wild-type SEC61B with a C-terminal sfGFP tag

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

SEC61B (SEC61B Human)

Use

Lentiviral

Tags

sfGFP

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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