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pJUMP18-sfGFP_O

Plasmid

#127018

Purpose

Basic Part O- ORF; sfGFP; fluorescent protein

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part sfGFP_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-B0034_R

Plasmid

#127012

Purpose

Basic Part R- RBS; B0034m Ribosome Binding Site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0034m_R

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-B0033_R

Plasmid

#127011

Purpose

Basic Part R- RBS; B0033m Ribosome Binding Site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0033m_R

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-B0032_R

Plasmid

#127010

Purpose

Basic Part R- RBS; B0032m Ribosome Binding Site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0032m_R

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Pmer_P

Plasmid

#127008

Purpose

Basic Part P- Promoter (non-constitutive); Pmer; metal sensitive; controlled by merR

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pmer_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Pars_P

Plasmid

#127007

Purpose

Basic Part P- Promoter (non-constitutive); Pars; metal sensitive; controlled by arsR

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pars_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Pci_P

Plasmid

#127006

Purpose

Basic Part P- Promoter (non-constitutive); Pci; controlled by cI.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pci_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-J23119s_P

Plasmid

#127001

Purpose

Basic Part P- Promoter (constitutive); J23119 (1bp short on 3').

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23119s_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-J23106_P

Plasmid

#126999

Purpose

Basic Part P- Promoter (constitutive); J23106.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23106_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-J23103_P

Plasmid

#126998

Purpose

Basic Part P- Promoter (constitutive); J23103.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23103_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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KO901: pMVP (L5-L4) Blast-P2A

Plasmid

#121715

Purpose

pMVP L5-L4 entry plasmid, contains Blast-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Blast selection marker linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Blast-P2A (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KP101: pMVP (L5-L4) Puro-P2A

Plasmid

#121716

Purpose

pMVP L5-L4 entry plasmid, contains Puro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Puro selection marker linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Puro-P2A (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KP001: pMVP (L5-L4) Neo-P2A

Plasmid

#121717

Purpose

pMVP L5-L4 entry plasmid, contains Neo-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Neo selection marker linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Neo-P2A (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KK802: pMVP (L5-L4) Zeo-P2A

Plasmid

#121719

Purpose

pMVP L5-L4 entry plasmid, contains Zeo-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Zeo selection marker linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Zeo-P2A (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IH001: pMVP (L5-L4) HA epitope tag

Plasmid

#121699

Purpose

pMVP L5-L4 entry plasmid, contains HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IH201: pMVP (L5-L4) 3x HA epitope tag

Plasmid

#121700

Purpose

pMVP L5-L4 entry plasmid, contains 3x HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

3x HA epitope tag (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IX203: pMVP (L5-L4) V5 epitope tag

Plasmid

#121705

Purpose

pMVP L5-L4 entry plasmid, contains V5 epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

V5 epitope tag (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KP401: pMVP (L5-L4) eCFP-P2A

Plasmid

#121712

Purpose

pMVP L5-L4 entry plasmid, contains eCFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eCFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP-P2A (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAP1071-2

Plasmid

#99502

Purpose

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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C077

Plasmid

#174346

Purpose

Template for making mRNA for overexpressing frog PABPC1 (C-FLAG) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (pabpc1.S Frog)

Use

In vitro transcription template

Tags

FLAG

Availability

Academic Institutions and Nonprofits only

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