We narrowed to 22,769 results for: Dos
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Plasmid#118599PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus sphingosine kinase 1
TagsFLAGExpressionYeastPromoterGAL1, 10Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIV303
Plasmid#204978PurposeAMA1 plasmid with Aspergillus optimized Mad7 and amdS selection markerDepositorInsertsMad7
amdS
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDIV303
Plasmid#204978PurposeAMA1 plasmid with Aspergillus optimized Mad7 and amdS selection markerDepositorInsertsMad7
amdS
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
3XFlag-TMCC1
Plasmid#121045PurposeExpresses human TMCC1 in mammalian cellsDepositorInsertTrans membrane and coiled coil domain containing protein 1 (TMCC1 Human)
Tags3x-FlagExpressionMammalianPromoterCMVAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
3XFlag-TMCC1
Plasmid#121045PurposeExpresses human TMCC1 in mammalian cellsDepositorInsertTrans membrane and coiled coil domain containing protein 1 (TMCC1 Human)
Tags3x-FlagExpressionMammalianPromoterCMVAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK1
Plasmid#118091PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorInsertHomo sapiens Sphingosine Kinase 1
TagsFLAGExpressionYeastPromoterGAL1, 10Available SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK1
Plasmid#118091PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorInsertHomo sapiens Sphingosine Kinase 1
TagsFLAGExpressionYeastPromoterGAL1, 10Available SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-eIF4E K119A
Plasmid#199440PurposeBacterial expression plasmids for production of recombinant GST-eIF4E K119ADepositorInsert1977 (EIF4E Human)
TagsGST-fusion proteinExpressionBacterialMutationchanged lysine 119 to alanineAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1-eIF4E K119A
Plasmid#199440PurposeBacterial expression plasmids for production of recombinant GST-eIF4E K119ADepositorInsert1977 (EIF4E Human)
TagsGST-fusion proteinExpressionBacterialMutationchanged lysine 119 to alanineAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
cASPG1-mRFP
Plasmid#181962PurposeASPG1 protein tagged with mRFP, under control of TBA2 promoterDepositorAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
cASPG1-mRFP
Plasmid#181962PurposeASPG1 protein tagged with mRFP, under control of TBA2 promoterDepositorAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ACTG1_Actin
Plasmid#109989PurposeProtein expression and purification of ACTG1_ActinDepositorInsertACTG1_Actin
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ACTG1_Actin
Plasmid#109989PurposeProtein expression and purification of ACTG1_ActinDepositorInsertACTG1_Actin
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry-CyrCR1.mutant
Plasmid#128746PurposeExpresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.DepositorInsertCyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
ExpressionMammalianMutation5 point mutations disrupting the miR-7 sitePromoterCMVAvailable SinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry-CyrCR1.mutant
Plasmid#128746PurposeExpresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.DepositorInsertCyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
ExpressionMammalianMutation5 point mutations disrupting the miR-7 sitePromoterCMVAvailable SinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-MinD_MinE
Plasmid#133621PurposeExpression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.DepositorAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-MinD_MinE
Plasmid#133621PurposeExpression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.DepositorAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOCC305
Plasmid#118898Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3CDepositorInsertNcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and TwinStrep-MB…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC305
Plasmid#118898Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3CDepositorInsertNcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and TwinStrep-MB…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -