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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,957 results for: pET

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Showing: 7301 - 7320 of 9957 results

  1. SDC229-B03

    Plasmid
    #58763
    Purpose
    bacterial expression of PDB 4PYZ
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4PYZ (USP7 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Aug. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. UHRF1

    Plasmid
    #25359
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh12.672.793 (UHRF1 Human)
    Tags
    His with thrombin cleavage site
    Expression
    Bacterial
    Mutation
    amino acid residues 672-793.
    Available since
    Dec. 21, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. KIF1C-FHA

    Plasmid
    #25549
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KIF1C-FHA (KIF1C Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    FHA domain only
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. Desmoplakin II donor only control (F40-based tension sensor)

    Plasmid
    #118717
    Purpose
    The donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.
    Depositor
    Carsten Grashoff
    Publication
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)-F40-mCherry(Y72L)] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118717-plasmid-map-sequence-id-230455

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  7. FLuc-N-HiBiT

    Plasmid
    #207117
    Purpose
    Bacterially expressed HiBiT-FLuc for Ni-NTA purification
    Depositor
    James Inglese
    Publication
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    luciferin 4-monooxygenase
    Tags
    HiBiT Tag and His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207117-plasmid-map-sequence-id-417172

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  10. 3JZN

    Plasmid
    #60123
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EED:APC056-C06:C32050 (EED Human, EED:APC056-C06:C32050)
    Tags
    6xHis and GST
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. pHL 1405

    Plasmid
    #53010
    Purpose
    pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Publication
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    DsrA sRNA
    hfq
    sodB
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains -56 to +141 relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. PRKCH

    Plasmid
    #25616
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PRKCH (PRKCH Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C2 domain (amino acid residues 1-138)
    Available since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pHybr_r2a>m2a(silent)-srt-his

    Plasmid
    #124807
    Purpose
    HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Publication
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2a (Fc silent)
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    L234A/L235A/N297A
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    124807-plasmid-map-sequence-id-251832

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  16. pAL612

    Plasmid
    #119860
    Purpose
    precusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 Cysteine
    Depositor
    Linda Randall
    Publication
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    outer membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
    Tags
    none
    Expression
    Bacterial
    Mutation
    Cysterine 290 to Serine and Glycine 244 to Cystei…
    Promoter
    lac
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119860-plasmid-map-sequence-id-232053

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  18. 4RG2

    Plasmid
    #64658
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    53BP1 (TP53BP1 Human)
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    April 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 7301 - 7320 of 9957 results