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Mechanical loading of desmosomes depends on the magnitude and orientation of external stress.
Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C
Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
118714Desmoplakin II-YPet(short)The human desmoplakin II is C-terminally tagged with YPet(short) to serve as wild type control.
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118715Desmoplakin II tension sensor (F40-based)The F40-based human desmoplakin II tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.
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118716Desmoplakin II no force control (F40-based)The no force control for the F40-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
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118717Desmoplakin II donor only control (F40-based tension sensor)The donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.
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118718Desmoplakin II donor only control (F40-based no force control)The donor (YPet(short)) only control for the no force control of the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.
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118719Desmoplakin II acceptor only control (F40-based tension sensor)The acceptor (mCherry) only control for the F40-based human desmoplakin II tension sensor can be co-expressed with the donor (YPet(short)) only control to determine intermolecular FRET.
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118720Desmoplakin II acceptor only control (F40-based no force control)The acceptor (mCherry) only control for the no force control of the F40-based human desmoplakin II tension sensor can be co-expressed with the donor only control to determine intermolecular FRET.
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118721Desmoplakin II tension sensor (FL-based)The FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry.
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118722Desmoplakin II donor only control (FL-based no force control)The donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.
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118723Desmoplakin II donor only control (FL-based tension sensor)The donor only (YPet(short)) control for the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.
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118724Desmoplakin II no force control (FL-based)The no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
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118725Desmoplakin I tension sensor (F40-based)The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.
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118726Desmoplakin I no force control (F40-based)The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
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119186Desmoplakin I tension sensor (F40-based)The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.
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119187Desmoplakin I no force control (F40-based)The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between mTFP1 and mEYFP that are tension-independent.
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119188Desmoplakin I donor only control (F40-based tension sensor)The donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.
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119189Desmoplakin I acceptor only control (F40-based tension sensor)The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.
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119190Desmoplakin I affinity mutant (F40-based tension sensor)The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.
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