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Desmoplakin II tension sensor (F40-based)
(Plasmid #118715)


Item Catalog # Description Quantity Price (USD)
Plasmid 118715 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 6265
  • Total vector size (bp) 13742
  • Modifications to backbone
    modified multiple cloning site
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy


  • Gene/Insert name
    human Desmoplakin II-[YPet(short)-F40-mCherry] (internal-1353)
  • Alt name
    Desmoplakin II tension sensor (F40-based)
  • Alt name
  • Alt name
    DPII-TS (YF40C)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    inserted F40-based tension sensor module after aa1353
  • GenBank ID
  • Entrez Gene
    DSP (a.k.a. DCWHKTA, DP)
  • Promoter CMV
  • Tag / Fusion Protein
    • YPet(short)-F40-mCherry

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ECoRI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer AGCTcGTTTAGTGAACCGTCAGATC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Desmoplakin II tension sensor (F40-based) was a gift from Carsten Grashoff (Addgene plasmid # 118715 ; ; RRID:Addgene_118715)
  • For your References section:

    Mechanical loading of desmosomes depends on the magnitude and orientation of external stress. Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C. Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0. 10.1038/s41467-018-07523-0 PubMed 30538252