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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,837 results for: pET

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Showing: 7541 - 7560 of 9837 results

  1. pAGH51

    Plasmid
    #107255
    Purpose
    HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.
    Depositor
    Erin O'Shea
    Article
    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111
    Insert
    HU-FRB
    Use
    Synthetic Biology
    Tags
    FRB
    Promoter
    native HU promoter
    Available Since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107255-plasmid-map-sequence-id-207562

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  2. pKK-mVenus-TEV

    Plasmid
    #105774
    Purpose
    Expression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mVenus-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105774-plasmid-map-sequence-id-203363

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  3. pfMDH_H303A

    Plasmid
    #67730
    Purpose
    Expresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed histidine 303 to alanine
    Promoter
    T7
    Available Since
    Sept. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pfMDH_D230A

    Plasmid
    #67725
    Purpose
    Expresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed aspartate 230 to alanine
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pfMDH_N191A

    Plasmid
    #67724
    Purpose
    Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed asparagine 191 to alanine
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pfMDH_K295A

    Plasmid
    #67726
    Purpose
    Expresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed lysine 295 to alanine
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. SULT1C2 (2AD1)

    Plasmid
    #25473
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SULT1C2 (SULT1C2 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. MAK3

    Plasmid
    #25499
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MAK3 (NAA50 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. EPHA2 (3KKA)

    Plasmid
    #25324
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    epha2.904.970 (EPHA2 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. KIF3B

    Plasmid
    #25254
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KIF3B:S6-Q359 (KIF3B Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids S6-Q359
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. pEB018

    Plasmid
    #215568
    Purpose
    Fuses insert of interest to an HA-tag, stop codon and S. commune Hom2 terminator, with flanks directing the construct to S. commune targeted integration locus
    Depositor
    Robin Ohm
    Insert
    3 x Hemagglutinin (HA) Tag / Stop codon / Intron / Hom2-terminator
    Use
    Fungal homologous recombination plasmid
    Available Since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    215568-plasmid-map-sequence-id-428736

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  19. FnR8-10

    Plasmid
    #182569
    Purpose
    Human fibronectin fragment, type-III repeats 8-10, R1379A mutant
    Depositor
    Timothy Springer
    Article
    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi
    Insert
    Human fibronectin fragment, type-III repeats 8-10
    Tags
    His and TEV cleavage site after His tag
    Expression
    Bacterial
    Mutation
    R1379A
    Available Since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182569-plasmid-map-sequence-id-357183

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Showing: 7541 - 7560 of 9837 results