We narrowed to 12,189 results for: LEA
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Plasmid#221717PurposeExpression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.DepositorInserthsN-drAgo2 (ago2 Zebrafish)
UseFish expressionTags3xFLAGMutationSwap of N-domain for human Ago2 N-domainPromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2F-Y
Plasmid#221722PurposeExpression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2F-Y (AGO2 Human)
UseFish expressionTags3xFLAGMutationF666Y in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2E-D
Plasmid#221721PurposeExpression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2E-D (AGO2 Human)
UseFish expressionTags3xFLAGMutationE637D in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2D-A
Plasmid#221720PurposeExpression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2D-A (AGO2 Human)
UseFish expressionTags3xFLAGMutationD669A in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24TEV-tSHIP2
Plasmid#183771PurposeExpresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backboneDepositorInsertSHIP2 (INPPL1 Human)
Tags6HIS and Tobacco Etch Virus (TEV) Cleavage siteExpressionBacterialMutationincludes aa18 to aa985 onlyPromoterT7 lacAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-CMV-SMCHD1-Exon37-46.47A-48-3xFLAG
Plasmid#130689PurposeExpresses human SMCHD1 Exon37-46.47A-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon37-46.47A-48 (SMCHD1 Human)
Tags3xFLAGExpressionMammalianMutation1M_T1522delinsM, M1961_E1992delPromoterCMVAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8_TtNAM-B1
Plasmid#124575PurposeTtNAM-B1 CDS in the Gateway-compatible pCR8 vector.DepositorInsertTaNAM-B1
UseTopo gateway cloning vectorPromoterNoneAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8_TaNAM-A1(1-217)
Plasmid#124576PurposeNAC domain (1-217) of TaNAM-A1 in the Gateway-compatible pCR8 vector.DepositorInsertTaNAM-A1 (1-217)
UseTopo gateway cloning vectorPromoterNoneAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-Hypa-nCas9n-A69
Plasmid#125645Purposemicro-injection of poly-adenylated Hypa-nCas9n RNADepositorInsertHypa-nCas9n
UseRna in vitro transcription vectorMutationN692A, M694A, Q695A, H698A from wild-type Cas9PromoterT3Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAL-SsoPCNA3
Plasmid#107069PurposeExpresses S. solfataricus PCNA3 fused to MBP in E. coliDepositorInsertS. solfataricus PCNA3
Tags6xHis-thrombin cleavage site and MBPExpressionBacterialPromotertac promoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3.2mar(T->G)-Luc
Plasmid#63697Purpose3.2 kb marine armor plate enhancer with a single T->G bp change driving Luciferase expressionDepositorInsert3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
TagsLuciferaseExpressionMammalianPromoterTATA boxAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ec FkP-dnaN(3E's)-pRSFDuet
Plasmid#238344Purposeover-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coliDepositorInsertPreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96E; R103E; R105E) (dnaN E. coli)
Tags3X FLAG+PKA kinase motif+PreScission Protease cle…ExpressionBacterialMutationR96E; R103E; R105EAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec FkP-dnaN(3A's)-pRSFDuet
Plasmid#238343Purposeover-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coliDepositorInsertPreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96A; R103A; R105A) (dnaN E. coli)
Tags3X FLAG+PKA kinase motif+PreScission Protease cle…ExpressionBacterialMutationR96A; R103A; R105AAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec FkP-dnaN(3S's)-pRSFDuet
Plasmid#238345Purposeover-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coliDepositorInsertPreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96S; R103S; R105S) (dnaN E. coli)
Tags3X FLAG+PKA kinase motif+PreScission Protease cle…ExpressionBacterialMutationR96S; R103S; R105SAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2EF-DY
Plasmid#221723PurposeExpression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2EF-DY (AGO2 Human)
UseFish expressionTags3xFLAGMutationE637D and F666Y in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
eZiPro
Plasmid#198434PurposeActivity/inhibition assays, 19F-NMRDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
TagsHis6-TEVExpressionBacterialPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-10A.12D-48-3xFLAG
Plasmid#130678PurposeExpresses human SMCHD1 Exon1-10A.12D-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-10A.12D-48 (SMCHD1 Human)
Tags3xFLAGExpressionMammalianMutationD409_F489delPromoterCMVAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046579
Plasmid#149092PurposeYeast Expression plasmid for SARS-CoV-2 3'-to-5' exonucleaseDepositorInsertSARS-CoV-2 3'-to-5' exonuclease (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046242
Plasmid#149047PurposeYeast Expression plasmid for SARS-CoV-2 3'-to-5' exonucleaseDepositorInsertSARS-CoV-2 3'-to-5' exonuclease (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046535
Plasmid#149033PurposeYeast Expression plasmid for SARS-CoV-2 3'-to-5' exonucleaseDepositorInsertSARS-CoV-2 3'-to-5' exonuclease (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits