We narrowed to 16,572 results for: Pol
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Plasmid#126423PurposeA kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)DepositorInsertcalcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
TagsFLAGExpressionMammalianMutationchanged lysine 75 to glutamic acidAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn3
Plasmid#173936PurposeBacterial expression of PARP1 lacking Zn3 domainDepositorInsertPARP1delZn3 (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 214-373; V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET32a-HD39Q
Plasmid#11514DepositorInserthuntingtin, exon 1, 39 glutamines (HTT Human)
TagsHis, His (out of frame), and TrxExpressionBacterialMutationhuntingtin exon 1 with 39 glutamines, vector m…Available SinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag MFF iso2 S172A
Plasmid#74392PurposeFlag tagged human S172A MFF isoform 2 mutantDepositorInsertFlag-MFF (MFF Human)
TagsFlagExpressionMammalianMutationSer 172 Ala (numbering based on MFF isoform 1)Available SinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2281
Plasmid#111902PurposessAAV genome with Ple346 (NEFM MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG
Plasmid#69842PurposeThis plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorInsertPLK4 (PLK4 Human)
Tags3xFLAG tag and EGFPExpressionMammalianMutationK41M-S285A-T289APromoterCMVAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Frt-hspB8
Plasmid#63099PurposeExpression of HSPB8 (small HSP) in mammalian cellsDepositorAvailable SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-mouseGIRK2-eGFP-StII-6xHis
Plasmid#124277PurposeExpresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tagDepositorAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn1
Plasmid#173933PurposeBacterial expression of PARP1 lacking Zn1 domainDepositorInsertPARP1delZn1 (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 1-96; V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-HD
Plasmid#174809PurposeBacterial expression of isolated PARP1 HD subdomainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract
Plasmid#138525PurposeExpresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.DepositorInsertControl non-PRC2 binding A-tract repeat RNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS-mTagRFP-TUBA1B
Plasmid#62850PurposeDonor vector for gene editing at human TUBA1B locusDepositorInsertmTagRFP-TUBA1B (TUBA1B Human, Synthetic)
UseYeast-e.coli shuttle vectorAvailable SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C entry vector
Plasmid#111752PurposeIntermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with C-term FLAG tag. Baculovirus transfer vector for insect and mammalian cellsDepositorTypeEmpty backboneUseBaculovirus expressionTagsFLAGExpressionMammalianAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEM-cytoBirA-2A-mCherry-SV40pA-FKF
Plasmid#79887PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ4M/TDP-43 S48E
Plasmid#104481Purposebacterial expression of TDP-43 S48EDepositorAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag GFP 4E-BP1 Rheb15
Plasmid#112761Purposeexpress GFP-4EBP1-Rheb15DepositorAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoEb
Plasmid#121901PurposePlasmid for making zebrafish apoEb in situ probeDepositorInsertApoeb (apoeb Zebrafish)
UseUnspecifiedAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract
Plasmid#138524PurposeExpresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.DepositorInsertPRC2-binding G-tract repeat RNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_NTD1-80_Y4R
Plasmid#104478Purposebacterial expression of TDP-43 NTD Y4RDepositorAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX131
Plasmid#167149PurposeMoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastorisDepositorInsert4'-phosphopantetheinyl transferase, npgA
UseSynthetic BiologyAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only