Showing: 1 - 20 of 7747 results
-
Plasmid#65520PurposeExpresses HBV polymerase in mammalian cellsDepositorInsertHBV polymerase protein
UseTagsFlagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLz54_polyPro_polar
Plasmid#140847PurposeSame as pLz47 but with 20 polar amino acids instead of N*- peptideDepositorInsertpolyproline helix with polar residues
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159676PurposePlasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.DepositorInsertpBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
UseTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159677PurposePlasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.DepositorInsert[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
UseTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 3 polH aeBlue polH VSV-G
Plasmid#206278PurposeENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.DepositorInsertaeBlue, VSV-G
UseMultimate/gateway entr 3TagsExpressionBacterial and InsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya
Plasmid#186980Purposemammalian expression of Cluc-Gluc reporterDepositorInsertCluc, Gluc
UseLuciferaseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA
Plasmid#168305Purposemediated knock-in of sgRNA precursorDepositorInsertSaKKH-EGFP-U6-sgRNA
UseAAVTagsExpressionMammalianMutationPromoterEF1alpha, CBhAvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)
Plasmid#208953PurposeA variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A)PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)
Plasmid#208956PurposeA variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
POLN_pDEST17
Plasmid#64885Purposebacterial expression of POLN for purificationDepositorInsertPOLN (POLN Human)
UseTagsFlag and HisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pSUP202pol4
Plasmid#67184PurposeConjugative cloning vectorDepositorTypeEmpty backboneUseConjugative bacterial cloning vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLLOXmPOLBeta
Plasmid#24174DepositorInsertpLLOXmPOLbeta (Polb Mouse)
UseLentiviralTagsExpressionMammalianMutationxPromoterAvailabilityAcademic Institutions and Nonprofits only -
PSL1180polyUBdsRED
Plasmid#49327PurposeDonor plasmid for homologous recombination that expresses dsRED constitutively using the Aedes aegypti polyubiquitin (PUb) promoter.DepositorInsertdsRED
UseTagsExpressionInsectMutationPromoterAedes polyubiquitinAvailabilityAcademic Institutions and Nonprofits only -
HO-Poly-HO
Plasmid#51661Purposeyeast plasmid for integration of target sequence at HO locusDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pOL2339
Plasmid#37839DepositorInsertUb-V
UseTagsmCherryExpressionWormMutationPromoternhx-2AvailabilityAcademic Institutions and Nonprofits only -
pOL2340
Plasmid#37841DepositorInsertUb-M
UseTagsmCherryExpressionWormMutationR77MPromoternhx-2AvailabilityAcademic Institutions and Nonprofits only -
pET11phiC31poly(A)
Plasmid#18942DepositorInsertphiC31 integrase (int Streptomyces phage phiC31)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
-
pDONR223_POLR2I_WT_V5
Plasmid#82955PurposeGateway Donor vector containing POLR2I, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertPOLR2I (POLR2I Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
POLD1_pLX307
Plasmid#98358PurposeLentiviral expression of POLD1DepositorInsertPOLD1 (POLD1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 7747 results