|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17531||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1 (Invitrogen)
- Backbone size w/o insert (bp) 2246
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHIV-1 Gag/Pol, Tat, Rev
Insert Size (bp)7274
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCD/NL-BH*DDD was a gift from Jakob Reiser (Addgene plasmid # 17531 ; http://n2t.net/addgene:17531 ; RRID:Addgene_17531)
For your References section:Transduction of bone-marrow-derived mesenchymal stem cells by using lentivirus vectors pseudotyped with modified RD114 envelope glycoproteins. Zhang XY, La Russa VF, Reiser J. J Virol. 2004 Feb . 78(3):1219-29. 10.1128/JVI.78.3.1219-1229.2004 PubMed 14722277