We narrowed to 947 results for: multisite gateway

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pME-H2B-mScarlet-3H (JDW 1513)

Plasmid

#242551

Purpose

Gateway middle entry clone containing H2B-mScarlet-3H; Nuclear red fluorescent reporter

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet3-H

Use

Gateway Cloning

Available since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Neo-DEST (JDW 940)

Plasmid

#229825

Purpose

A neo selectable, PiggyBac destination vector compatible with gateway cloning system.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-hs_ICAM_full (JDW 1047)

Plasmid

#224479

Purpose

Gateway compatible 5' entry clone containing the Human ICAM2 promoter

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

ICAM2 promoter

Expression

Mammalian

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-myc-BirA (JDW 1210)

Plasmid

#224509

Purpose

Gateway compatible middle entry clone containing a Myc tagged BirA

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Myc-BirA

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)

Plasmid

#224532

Purpose

A Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyA

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-H2A-mCherry-SV40pA

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-(n2)StayGold(c4)-pA (JDW 1364)

Plasmid

#224537

Purpose

A Gateway compatible 3' entry clone containing IRES-(n2)StayGold(c4)-pA

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-(n2)StayGold(c4)-pA

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pB-EF1a-FastFUCCI-IRES-3xnls-mTagBFP2 (JDW 1511)

Plasmid

#242599

Purpose

A PiggyBac expression vector containing the human EF1a promoter driving FastFUCCI to label cell cycle state followed by an IRES-3xnls-mTagBFP2.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110) (EEF1A1 Human)

Expression

Mammalian

Promoter

human EF1a

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Actin-Vhh-mNeonGreen-HA-pA (JDW 1308)

Plasmid

#242589

Purpose

A CAGGS driven expression vector containing an actin nanobody fused to mNeonGreen to label actin.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh (actin nanobody)

Expression

Mammalian

Promoter

CAGGS

Available since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)

Plasmid

#229845

Purpose

A CAGGS-driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling followed by an IRES and a myristoylated TagBFP for labeling the cell membrane.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lamin nanobody

Expression

Mammalian

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-zFucci-pA-exorh-GFP (JDW 1489)

Plasmid

#242593

Purpose

A Tol2 expression vector containing the zebrafish Ubi promoter driving zFUCCI to label cell cycle state. Exorh-EGFP in the backbone to label pineal cells.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin, mCherry-zCdt1

Use

Zebrafish expressio

Promoter

zebrafish Ubi

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN-DV-IRES-myr-EGFP (JDW 495)

Plasmid

#242574

Purpose

Gateway destination vector with a CAGGS promoter in the backbone as well as an IRES-myr-EGFP to label cell membranes.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attR1-CmR-ccdB-attR2

Expression

Mammalian

Promoter

CAGGS

Available since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Puro-DEST (JDW 925)

Plasmid

#229808

Purpose

A puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning system

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-WPRE-bGH-DEST (JDW 1309)

Plasmid

#229815

Purpose

A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGLAST-DEST (JDW 1009)

Plasmid

#229822

Purpose

A single site gateway compatible destination vector with an upstream GLAST promotor to drive expression in glial cells.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBeta-MHC-promoter DV (JDW 26)

Plasmid

#229835

Purpose

A gateway compatible destination vector containing the murine beta-MHC promoter for embryonic and postnatal cardiomyocyte expression.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-MCS-c-Fos-b-globin (JDW 1237)

Plasmid

#229829

Purpose

A gateway compatible 5' entry clone containing an MCS upstream of the murine c-fos minimal promotor and b-globin intron.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

c-fos minimal promoter and b-globin intron

Use

Gateway Cloning

Expression

Mammalian

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)

Plasmid

#224490

Purpose

Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Golgi-mTagBFP-P2A-H2A-iRFP

Use

Gateway Cloning

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pME-rox-nls-mCherry-V5-2xStop-rox (JDW 1167)

Plasmid

#224524

Purpose

A Gateway compatible middle entry clone containing a Rox flanked 3xNLS-mCherry-V5-2xStop cassette

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

rox-3xNLS-mCherry-V5-2xSTOP-rox

Use

Gateway Cloning

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pME V5-mTagBFP2 (JDW 830)

Plasmid

#224484

Purpose

Gateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mTagBFP2

Use

Gateway Cloning

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-H2B-V5-n2StayGoldC4 (JDW 1354)

Plasmid

#224486

Purpose

Gateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-V5-n2StayGoldC4

Use

Gateway Cloning

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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