We narrowed to 15,991 results for: LEA

1. Helios-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75246

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human Helios (1/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Helios (IKZF2 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Helios-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75247

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human Helios (2/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Helios (IKZF2 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. si:ch211-221n23.1_R (OZ604)

   Plasmid

   #35244

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting si:ch211-221n23.1 (si:ch211-221n23.1 Zebrafish)
   

   Use

   Zebrafish targeting

   Available Since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

4. si:ch211-221n23.1_L (OZ603)

   Plasmid

   #35243

   Depositor

   Keith Joung

   Article

   Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

   Insert

   Zinc finger array targeting si:ch211-221n23.1 (si:ch211-221n23.1 Zebrafish)
   

   Use

   Zebrafish targeting

   Available Since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

   Plasmid

   #179106

   Purpose

   pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3Basic-ME.2/ApoEpromoter

   Plasmid

   #51436

   Purpose

   This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancer

   Depositor

   Sohail Tavazoie

   Article

   Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

   Insert

   APOE apolipoprotein E (APOE, ME2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   The ME.2 enhancer is fused upstream of the ApoE g…

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

   Plasmid

   #217800

   Purpose

   Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Plasmid

   #179105

   Purpose

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

   Plasmid

   #208959

   Purpose

   A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

    Plasmid

    #125712

    Purpose

    Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    eOPN3
    

    Use

    AAV

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    deleted amino acids 331-429

    Promoter

    short version of αCamKinase II promoter (0.4kb)

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)

    Plasmid

    #179115

    Purpose

    Cargo EGFP with AttP Bxb1 site (Parent minicircle)

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    Cargo EGFP with AttP Bxb1 site
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac1-pGC-A

    Plasmid

    #186626

    Purpose

    pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)

    Depositor

    Debra Hansen

    Article

    Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1

    Insert

    NPR1 (NPR1 Human)
    

    Tags

    hemagglutinin (HA) signal peptide + His10-tag + T…

    Expression

    Insect

    Mutation

    deleted amino acids 1-32

    Promoter

    polyhedring

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

    Plasmid

    #125713

    Purpose

    Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

    Has Service

    AAV1 and AAV5

    Insert

    eOPN3
    

    Use

    AAV and Cre/Lox

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    deleted amino acids 331-429

    Promoter

    hSyn1

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

    Plasmid

    #179116

    Purpose

    Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    Cargo EGFP with AttP Bxb1 site for in-frame fusion
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron

    Plasmid

    #235086

    Purpose

    GFP splicing reporter with CORO1B intron

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron (CORO1B Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    custom bidirectional

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRIPZ-bioGEF-UBnc-PURO

    Plasmid

    #208044

    Purpose

    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    UBnc (UBC Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Mutation

    L73P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGJK_His-SUMO-LbuCas13a

    Plasmid

    #172488

    Purpose

    Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    bdSUMO-LbuCas13a (codon-optimized)
    

    Tags

    His6 and bdSUMO

    Expression

    Bacterial

    Promoter

    T7 promoter and lac operator

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron

    Plasmid

    #235087

    Purpose

    GFP splicing reporter with FRG1 intron

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron (FRG1 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    custom bidirectional

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid

    #217808

    Purpose

    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. peGFP-N1-Cofilin

    Plasmid

    #186747

    Purpose

    Expresses wildtype GFP-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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