We narrowed to 17,548 results for: emb

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3xMS2 TR knockin sgRNA 1

Plasmid

#207607

Purpose

sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

cgactcgcccggcagcgcac

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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3xMS2 TR knockin sgRNA 2

Plasmid

#207608

Purpose

sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

acccccaaacctgactgact

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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TR knockout HRD

Plasmid

#207579

Purpose

Homologous recombination donor replacing TR with a puro cassette.

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

puro resistance cassette flanked by TR locus sequences excluding TR

Tags

None

Expression

Mammalian

Promoter

SV40

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Duet_OPTN-thrombin-GST

Plasmid

#216843

Purpose

For expression in E. coli of OPTN (Internal ref. SMC1789)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

Optineurin (OPTN Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TST-EGFP-GPIDAF

Plasmid

#213712

Purpose

For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag and V5-tag

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TST-EGFP-GPIBY55

Plasmid

#213711

Purpose

For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag and V5-tag

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLCS100

Plasmid

#209354

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal YFP fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pUBQ10-HALO-GW

Plasmid

#209351

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal HaloTag fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLCS103

Plasmid

#209356

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal SNAP-tag fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pJRA106

Plasmid

#209339

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal moxMaple fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJRA101

Plasmid

#209335

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mVenus fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk2a

Plasmid

#208983

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk2a (kcnk2a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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RGG-BcLOV-mCh-SOS

Plasmid

#208278

Purpose

Expresses RGG-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

RGG-BcLOV-mCh-SOS

Use

Lentiviral

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh-EGFR

Plasmid

#208281

Purpose

Expresses Fus-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh-EGFR

Use

Lentiviral

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLP-sfgfp

Plasmid

#209994

Purpose

Contains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

sfGFP

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPT1

Plasmid

#209995

Purpose

Contains Level 0 Part: Terminator (TB1006) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

Terminator (TB1006)

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPS1

Plasmid

#209992

Purpose

Contains Level 0 Part: Ribosome binding site (RB0034) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

RBS (RB0034)

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKE011

Plasmid

#207912

Purpose

Level 0 for gRNA in C4 module (5' GCAG-3' TCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETmsGFPSyt1(80 JuxtaK-142)

Plasmid

#209013

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis-msfGFP

Expression

Bacterial

Mutation

aa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETSyt1C2AB(143-421)msGFP

Plasmid

#209010

Purpose

Recombinant protein expression of truncated syt1 tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis and msfGFP

Expression

Bacterial

Mutation

aa 143-421 only

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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