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pcDNA5-FRT-TO-FH-Orf6

Plasmid

#157727

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Orf6 (ORF6 SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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DNAJC11-EGFP

Plasmid

#210368

Purpose

Expresses DNAJC11 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

DNAJC11 (DNAJC11 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-CRY2

Plasmid

#140572

Purpose

For use in light-induced protein inactivation through the interaction with CIB1

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSI-check2-CUL1-3'UTR

Plasmid

#97156

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

CUL1 3'UTR (CUL1 Human)

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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NSP14-Flag

Plasmid

#210346

Purpose

Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pQTEV-AIP

Plasmid

#31575

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

aryl hydrocarbon receptor interacting protein (AIP Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-NOS-IN133.3xFLAG.mCherry-WPRE

Plasmid

#127868

Purpose

pAAV plasmid expressing an NOS-IN133.3xFLAG.mCherry fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1 (Nos1 Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

Amino acids 1-133 only

Promoter

hSyn

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR insertionH

Plasmid

#32067

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Insert H at H773-V774

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgNCKAP1-2

Plasmid

#210134

Purpose

knock out NCKAP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Nck-associated protein 1 (NCKAP1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgNCKAP1-1

Plasmid

#210133

Purpose

knock out NCKAP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Nck-associated protein 1 (NCKAP1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-Tubg1-mNeonGreen

Plasmid

#196869

Purpose

Expression of the gamma-tubulin fused to mNeonGreen

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Tubg1-mNeonGreen (Tubg1 Mouse)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp8-HF

Plasmid

#157696

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp7

Plasmid

#157688

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp7 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBabe EGFR Del2

Plasmid

#32065

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Delete S752-I759

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
LN-entry+NLS

Plasmid

#128011

Purpose

Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

Depositor

Julius Brennecke

Article

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

LN-3xFlag-SV40NLS

Use

Lamdan entry vector with nls to generate tetherin…

Tags

LN-3xFlag-SV40NLS

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3T911A

Plasmid

#120254

Purpose

Expression of ATP3-6 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Expression

Yeast

Mutation

T911 changed to A; Isoleucine 304 changed to Aspa…

Promoter

ATP3

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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ABE8.8-d

Plasmid

#136295

Purpose

expresses ABE8.8-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.8-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-sACt-mCherry

Plasmid

#138216

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

Lyn-sACt-mCherry (Adcy10 Rat)

Tags

N-terminal targeting sequence from Lyn kinase and…

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG GST DEPDC5

Plasmid

#164983

Purpose

DEPDC5 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

DEPDC5 (DEPDC5 Human)

Tags

GST

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE)

Plasmid

#214877

Purpose

Lentiviral vector encoding RfxCas13d targeting LPT guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crLPT-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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