Yeast MoClo secretion and surface display toolkit 2.0: improvements and applications for analysis of protein-protein interactions and whole-cell biocatalysis
Jurić V, Erwin LG, O'Riordan NM, Maher E, Holmes JD, Young PW
bioRxiv 2025.08.19.671047
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 252961 | pYSD045 | Encodes S. cerevisiae Pir anchor-linker-HA Tag as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252962 | pYSD046 | Encodes S. cerevisiae Pir anchor-linker-2xFLAG Tag as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252963 | pYSD047 | Encodes S. cerevisiae Pir2 anchor-linker-MYC Tag as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252964 | pYSD051 | Encodes S. cerevisiae Aga2-(NoTag)-linker as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2 |
| 252965 | pYSD052 | Encodes S. cerevisiae HA Tag-Aga2-linker as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2 |
| 252966 | pYSD053 | Encodes S. cerevisiae 2xFLAG-Tag-Aga2-linker as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2 |
| 252967 | pYSD054 | Encodes S. cerevisiae MYC Tag-Aga2-linker as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2 |
| 252968 | pYSD055 | Encodes S. cerevisiae Aga2-(NoTag)-linker as a Type 3a part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2 |
| 252969 | pYSD056 | Encodes S. cerevisiae HA Tag-Aga2-linker as a Type 3a part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2 |
| 252970 | pYSD057 | Encodes S. cerevisiae 2xFLAG-Tag-Aga2-linker as a Type 3a part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2 |
| 252971 | pYSD058 | Encodes S. cerevisiae MYC Tag-Aga2-linker as a Type 3a part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2 |
| 252973 | pYSD069 | Encodes Anti-GFP Nanobody (no tag) as a Type 3b' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252974 | pYSD070 | Encodes Protein A ZZ domain as a Type 3b' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252978 | pYSD088 | Encodes 2xFLAG-Tagged S. cerevisiae Aga2p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit for N-terminal fusion |
| 252981 | pYSD091 | Encodes S. cerevisiae HA-tagged Aga1 del N181aa disp anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252982 | pYSD092 | Encodes S. cerevisiae 2xFLAG-tagged Aga1 del N181aa disp anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252983 | pYSD093 | Encodes MYC-tagged S. cerevisiae Aga1 del N181aa disp anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252984 | pYSD094 | Encodes GFP11-M3-His6-tag as a Type 4a part to be used for fluoresence complementation based detection in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252985 | pYSD095 | Encodes GFP11-M3 as a Type 4a part to be used for fluoresence complementation based detection in the yeast secretion/display (YSD2.0) MoClo toolkit Fluoresence Complementation |
| 252988 | pYSD503 | Encodes S. cerevisiae ERV29 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252989 | pYSD504 | Encodes S. cerevisiae ERJ5 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252990 | pYSD505 | Encodes S. cerevisiae ERO1 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252991 | pYSD506 | Encodes S. cerevisiae HAC1 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252992 | pYSD507 | Encodes S. cerevisiae MSN4 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252993 | pYSD508 | Encodes S. cerevisiae PDI1 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252994 | pYSD509 | Encodes S. cerevisiae SEB1 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252995 | pYSD510 | Encodes S. cerevisiae SIL1 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252996 | pYSD511 | Encodes S. cerevisiae SSO1 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252997 | pYSD512 | Encodes S. cerevisiae SSO2 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252998 | pYSD513 | Encodes S. cerevisiae ERV2 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 252999 | pYSD514 | Encodes S. cerevisiae Sec65 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 253000 | pYSD515 | Encodes S. cerevisiae SRP14 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 253001 | pYSD516 | Encodes S. cerevisiae SWA2 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 253002 | pYSD517 | Encodes S. cerevisiae COG5 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 253004 | pYSD519 | Encodes S. cerevisiae SRP54 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 253005 | pYSD520 | Encodes S. cerevisiae MNS1 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 253006 | pYSD521 | Encodes S. cerevisiae SEC4 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 253007 | pYSD522 | Encodes S. cerevisiae CWH41 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 253008 | pYSD523 | Encodes S. cerevisiae SEC16 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 253009 | pYSD524 | Encodes S. cerevisiae CYS4 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 253010 | pYSD525 | Encodes S. cerevisiae SED5 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 253011 | pYSD526 | Encodes S. cerevisiae JEM1 as a Type 3 part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 253012 | pYSD703 | Encodes Ideonella sakaiensis MHETase as a Type 3b' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 253014 | pYSD861 | Encodes Anti-ALFA-tag Nanobody as a Type 3b' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 253015 | pYSD862 | Encodes Anti-mCherryLaM-4 Nanobody as a Type 3b' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK) |
| 253019 | pYSD893 | A MoClo YTK Type 3 part GFP dropout vector to assemble a signal peptide and protein of interest as N-terminal fusion with 2xFLAG-SED1 display anchor in a URA3, CEN6/ARS4 yeast expression plasmid |
| 253021 | pYSD274 | Encodes the Aga2 signal peptide, mRuby2 fluorescent protein, C-terminal GFP11-M3 and 6xHistidine tags in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker |
| 253022 | pYSD281 | Encodes the Suc2 signal peptide, Invertase, C-terminal GFP11-M3 and 6xHistidine tags in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker |
| 253023 | pYSD288 | Encodes the S. cerevisiae Aga1 protein (Part3) with pGAL1 inducible promoter, tENO2 terminator, KanamycinR yeast selectable marker and CEN/ARS yeast origin |
| 253024 | pYSD291 | Encodes the S. cerevisiae Aga1 protein (Part3) with pPGK1 promoter, tENO2 terminator, KanamycinR yeast selectable marker and CEN/ARS yeast origin |
| 253025 | pYSD292 | Encodes the S. cerevisiae Aga1 protein (Part3) with pPGK1 promoter, tENO2 terminator, HygromycinR yeast selectable marker and CEN/ARS yeast origin |
| 253026 | pYSD293 | Encodes the S. cerevisiae Aga1 protein (Part3) with ppPGK1 promoter, tENO2 terminator, ZeocinR yeast selectable marker and CEN/ARS yeast origin |
| 253028 | pYSD295 | Encodes the S. cerevisiae Aga1 protein (Part3) with pRPL18B promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin |
| 253030 | pYSD299 | Encodes the S. cerevisiae Aga1 protein (Part3) with pPGK1 promoter, tENO2 terminator, LEU2 selectable marker and CEN/ARS yeast origin |
| 253031 | pYSD301 | Encodes the Aga2 signal peptide, anti GFP nanobody, HA-tagged Aga2p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin |
| 253032 | pYSD310 | Encodes the MFalpha1 PrePro-app8 signal peptide, Anti-GFP nanobody, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker |
| 253033 | pYSD311 | Encodes the Aga2 signal peptide, mRuby2 fluorescent protein, HA-tagged Aga2p yeast surface display anchor protein with pTEF1 promoter, LEU2 selectable marker |
| 253034 | pYSD315 | Encodes the Aga2 signal peptide, mRuby2 fluorescent protein, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker |
| 253035 | pYSD316 | Encodes the MFalpha1 PrePro-app8 signal peptide, Anti-GFP nanobody, HA-tagged Aga1 deltaN181AA yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker |
| 253037 | pYSD322 | Encodes the MFalpha1 PrePro-app8 signal peptide, Anti-HA Frankenbody, MYC-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker |
| 253038 | pYSD325 | Encodes the MFalpha1 PrePro-app8 signal peptide, Protein A ZZ domain, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker |
| 253039 | pYSD326 | Encodes the OST1-pre / MFalpha1 Pro signal peptide, Protein A ZZ domain, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker |
| 253041 | pYSD329 | Encodes the Aga2 signal peptide, anti-mCherry_LaM4 nanobody, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker |
| 253042 | pYSD332 | Encodes HA-tagged Aga2 yeast surface display anchor protein, Anti-GFP nanobody as a C-terminal fusion, in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker |
| 253043 | pYSD335 | Encodes HA-tagged Aga2 with mRuby2 as a C-terminal fusion in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker. |
| 253045 | pYSD353 | Encodes the MFalpha1 PrePro-app8 signal peptide, Anti-GFP nanobody, FLAG-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker |
| 253046 | pYSD354 | Encodes the MFalpha1 PrePro-app8 signal peptide, Anti-GFP nanobody, MYC-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker |
| 253047 | pYSD371 | Encodes an AGA2 Signal Peptide, Anti- ALFAtag_nanobody and 2xFLAG-tagged-649-stalk anchor in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker |
| 253048 | pYSD373 | Encodes an AGA2 Signal Peptide, Anti-mCherry_nanobody_LaM-4 and 2xFLAG-tagged-649-stalk anchor in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker |