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pYSD301
(Plasmid #253031)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253031 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pYTK083
  • Backbone manufacturer
    John Dueber, Addgene plasmid #65190
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Anti-GFP nanobody -Aga2
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYSD301 was a gift from Paul Young (Addgene plasmid # 253031 ; http://n2t.net/addgene:253031 ; RRID:Addgene_253031)

  • For your References section:

    Yeast MoClo secretion and surface display toolkit 2.0: improvements and applications for analysis of protein-protein interactions and whole-cell biocatalysis. Jurić V, Erwin LG, O'Riordan NM, Maher E, Holmes JD, Young PW. bioRxiv 2025.08.19.671047 10.1101/2025.08.19.671047
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