pYSD055
(Plasmid
#252968)
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PurposeEncodes S. cerevisiae Aga2-(NoTag)-linker as a Type 3a part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 252968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepYTK001
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Backbone manufacturerJohn Dueber, Addgene plasmid #65108
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAga2
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Golden Gate
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYSD055 was a gift from Paul Young (Addgene plasmid # 252968 ; http://n2t.net/addgene:252968 ; RRID:Addgene_252968) -
For your References section:
Yeast MoClo secretion and surface display toolkit 2.0: improvements and applications for analysis of protein-protein interactions and whole-cell biocatalysis. Jurić V, Erwin LG, O'Riordan NM, Maher E, Holmes JD, Young PW. bioRxiv 2025.08.19.671047 10.1101/2025.08.19.671047