We narrowed to 59,446 results for: cloning

1. pENTR-FLAG-hDIXDC1-L-WT

   Plasmid

   #61210

   Purpose

   Entry clone encoding long isoform of human DIXDC1

   Depositor

   Reuben Shaw

   Article

   Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

   Insert

   human DIXDC1 (DIXDC1 Human)
   

   Use

   Entr clone

   Tags

   FLAG

   Promoter

   None

   Available Since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pENTR223-BORF1

   Plasmid

   #195804

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BORF1

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BORF1
   

   Use

   Gateway entry vector

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA flag-Msi1

   Plasmid

   #184029

   Purpose

   Msi1 clone with N-terminal flag tag

   Depositor

   Peter Stoilov

   Article

   Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

   Insert

   Msi1 (Msi1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMU-4

   Plasmid

   #172353

   Purpose

   CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA

   Depositor

   Eriko Takano

   Article

   Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

   Insert

   D4-TheoR-cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   D4

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCPP5951

   Plasmid

   #128719

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

   Insert

   hopR1
   

   Expression

   Bacterial

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV5 MEKK4alpha WT

   Plasmid

   #12187

   Depositor

   Gary Johnson

   Article

   Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

   Insert

   MEKK4 (Map3k4 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-CMV-SauriCas9-Puro

   Plasmid

   #135965

   Purpose

   Expresses SauriCas9 and puromycin resistance genes, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBEG R2-dsRed-L3 #BGV203

   Plasmid

   #48973

   Purpose

   Supplies the selection marker (dsRed) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   dsRed
   

   Use

   Gateway cloning vector

   Tags

   IRES (strong)

   Promoter

   none

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pU6-Universal.Sth.dCas9

   Plasmid

   #171088

   Purpose

   CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
   DHFR-TSc3
   

   Use

   Toxoplasma gondii expression

   Mutation

   Note: A S245F mutation is present but did not app…

   Promoter

   DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. UKNP6.1.1

    Plasmid

    #98386

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. E2F-1 wt-pGex2TK

    Plasmid

    #21668

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

    Insert

    E2F-1 (E2F1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    wild type

    Available Since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO HIS HSPA1L

    Plasmid

    #19538

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 1-like (HSPA1L Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO GFP DNAJB2b

    Plasmid

    #19496

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB2b (DNAJB2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEG R2-iBlast-L3 #BGV204

    Plasmid

    #48982

    Purpose

    Supplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Insert

    blasticidin-S deaminase
    

    Use

    Gateway cloning vector

    Tags

    IRES (weak)

    Promoter

    na

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pUC19-TSV (linearized in LT)

    Plasmid

    #70033

    Purpose

    pUC19-TSV (linearized in LT) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the Large T-antigen coding region

    Depositor

    Mariet Feltkamp

    Article

    van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.

    Insert

    TSPyV full genome, linearized in Large T-antigen
    

    Use

    Cloning vector

    Expression

    Bacterial

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. B270 + SPRTN sgSTOP

    Plasmid

    #100718

    Purpose

    B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting SPRTN (cloned using BbsI)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDSRaD-sdi1

    Plasmid

    #219566

    Purpose

    Mammalian expression of human sdi1 (p21 CDK inhibitor)

    Depositor

    Asao Noda, James R Smith

    Article

    Noda et al Exp Cell Res. 1994 Mar;211(1):90-8. doi:

    Insert

    sdi1 (CDKN1A Human)
    

    Expression

    Mammalian

    Promoter

    SRa

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBEG R3-miRNA(ccdB)-L4 #BGV228

    Plasmid

    #48953

    Purpose

    To create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Gateway cloning vector

    Promoter

    na

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pBEG R2-iHygro-L3 #BGV206

    Plasmid

    #48985

    Purpose

    Supplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Insert

    hygromycin phosphotransferase
    

    Use

    Gateway cloning vector

    Tags

    IRES (weak)

    Promoter

    na

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pBS hIhh (CT#402)

    Plasmid

    #13995

    Depositor

    Cliff Tabin

    Article

    Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

    Insert

    Ihh (IHH Human)
    

    Expression

    Bacterial

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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