We narrowed to 32,758 results for: eng

1. pCDNA5/FRT/TO_HaloTag9_T2A_EGFP

   Plasmid

   #169326

   Purpose

   Expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-Fos-CreERT2

   Plasmid

   #194643

   Purpose

   Expresses Cre-ERT2 under the control of c-Fos promoter

   Depositor

   Jun-Hyeong Cho

   Article

   Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

   Insert

   Fos-CreERT2
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBABE-puro SV40 LT

   Plasmid

   #13970

   Depositor

   Thomas Roberts

   Article

   Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

   Insert

   SV40 LT (SV40gp6 )
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PCDNA3-b2AR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

   Plasmid

   #113610

   Purpose

   PPI reporter

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

   Insert

   beta 2 adrenergic receptor
   

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EJ2GFP-puro

   Plasmid

   #44025

   Depositor

   Jeremy Stark

   Article

   Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

   Insert

   EJ2GFP egfp-based chromosomal break reporter
   

   Expression

   Mammalian

   Mutation

   insertion of I-SceI, 3 frame stop, and flanking m…

   Promoter

   pCAGGS

   Available Since

   May 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Mito-mEosEM

   Plasmid

   #132706

   Purpose

   application in Correlative light-electron microscopy

   Depositor

   Pingyong Xu

   Article

   Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   mitochondria targeting sequence
   

   Tags

   mEosEM

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCR3.1-OPNa

   Plasmid

   #107980

   Purpose

   expression of full-length OPN

   Depositor

   Georg Weber

   Article

   He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

   Insert

   full-length osteopontin (SPP1 Human)
   

   Expression

   Mammalian

   Available Since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. synIRES_RC25

   Plasmid

   #189970

   Purpose

   Part 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAs

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   synIRES_RC25
   

   Use

   Cloning

   Promoter

   N/A

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mEosEM-Lamin A

   Plasmid

   #132707

   Purpose

   application in Correlative light-electron microscopy

   Depositor

   Pingyong Xu

   Article

   Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   lamin A
   

   Tags

   mEosEM

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7RNAP

    Plasmid

    #170101

    Purpose

    expresses T7 RNAP

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    T7-RNA polymerase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    p70a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PX458-AAVS1

    Plasmid

    #113194

    Purpose

    Encodes gRNA for human AAVS1

    Depositor

    Adam Karpf

    Insert

    AAVS1 (PPP1R12C, AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHR_5x Gal4 UAS

    Plasmid

    #79119

    Purpose

    Base vector for cloning Gal4DBDVP64 inducible genes

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RepX_pET28a(+)

    Plasmid

    #172199

    Purpose

    Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

    Depositor

    Taekjip Ha

    Article

    Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

    Insert

    RepX superhelicase
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    C18L, C43S, C167V, S400C, C612A

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEA186

    Plasmid

    #187874

    Purpose

    Empty pVS1-based INTEGRATE vector with mCherry cargo

    Depositor

    Kan Wang

    Article

    Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPRyl_XPR2

    Plasmid

    #84609

    Purpose

    Introduces DSB at XPR2 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRISPRyl_AXP

    Plasmid

    #84608

    Purpose

    Introduces DSB at AXP locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPRyl_D17

    Plasmid

    #84611

    Purpose

    Introduces DSB at D17 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPRyl_A08

    Plasmid

    #84610

    Purpose

    Introduces DSB at A08 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPRyl_MFE1

    Plasmid

    #84612

    Purpose

    Introduces DSB at MFE1 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pACYC-Skp-GroEL/ES

    Plasmid

    #83922

    Purpose

    Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Skp
    Gro EL/ES
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge