We narrowed to 15,991 results for: LEA

1. pcDNA3.1-GSDMD_D275A

   Plasmid

   #224704

   Purpose

   Expression of mutated Gasdermin D_D275A (GSDMD_D275A) in transfected mammalian cell lines

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD_D275A (GSDMD Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D275A

   Promoter

   CVM

   Available Since

   July 2, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3.1-GSDMD_H270A_D275A

   Plasmid

   #224705

   Purpose

   Expression of mutated Gasdermin D_H270A_D275A (GSDMD_D275A) in transfected mammalian cell lines

   Depositor

   Christopher Overall

   Article

   Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

   Insert

   GSDMD_Q29A_H270A (GSDMD Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H270A and D275A

   Promoter

   CMV

   Available Since

   July 2, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCAG-MCP-Lbr-V5-mCherry

   Plasmid

   #235093

   Purpose

   Lbr mCherry fusion protein with MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-MCP-Lbr-V5-mCherry (LBR Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBIG1e-CDK12deltaNTD /Cyclin K

   Plasmid

   #231741

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Inserts

   CDK12 (CDK12 Human)
   CCNK (CCNK Human)
   

   Tags

   Flag-3C

   Expression

   Insect

   Mutation

   CDK12(700-1490)

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBIG1e-CDK12deltaCTD/Cyclin K

   Plasmid

   #231742

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Inserts

   CDK12 (CDK12 Human)
   CCNK (CCNK Human)
   

   Tags

   Flag-3C

   Expression

   Insect

   Mutation

   CDK12(1-1082)

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBIG1e-CDK12-KiD/Cyclin K

   Plasmid

   #231743

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Inserts

   CDK12 (CDK12 Human)
   CCNK (CCNK Human)
   

   Tags

   Flag-3C

   Expression

   Insect

   Mutation

   CDK12(700-1082)

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-SpCas9-P2A-EGFP (MSP2582)

   Plasmid

   #223067

   Purpose

   pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized SpCas9-P2A-EGFP
   

   Use

   CRISPR

   Tags

   NLS(SV40)-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-multi-CRISPR-sgRipk1_#1-puro

   Plasmid

   #231984

   Purpose

   Knockout mouse Ripk1

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-multi-CRISPR-sgRipk1_#2-puro

   Plasmid

   #231983

   Purpose

   Knockout mouse Ripk1

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3xFLAG-DGKδ2-ΔSAMD

    Plasmid

    #226507

    Purpose

    Express N-terminal three tandem FLAG epitope tags, followed by an enterokinase cleavage site and human DGKD gene deleted SAM domain

    Depositor

    Fumio Sakane, Chiaki Murakami

    Article

    Murakami et al J Biol Chem. 2020 Mar 6;295(10):2932-294

    Insert

    DGKD (DGKD Human)
    

    Tags

    Three tandem FLAG epitope tag and enterokinase cl…

    Expression

    Mammalian

    Mutation

    deleted sterile alpha motif domain (deleted amino…

    Promoter

    CMV

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-FRB:GSlinker:SSD

    Plasmid

    #228535

    Purpose

    FRB protein in fusion with a bipartite SSD degron, consisting of fragments of human Skp1 and SOCS2 proteins, through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    FRB:GSlinker:SSD (SKP1, SOCS2 Human, Synthetic)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cdk13_intron3_sg4_pX458

    Plasmid

    #127339

    Purpose

    sgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #4

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Mouse Cdk13 Intron 3 sgRNA (Cdk13 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Promoter

    U6 Promoter

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cdk13_intron4_sg3_pX330

    Plasmid

    #127340

    Purpose

    sgRNA that cuts within intron 4 of mouse CDK13 genomic locus- guide #3

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Mouse Cdk13 Intron 4 Targeting sgRNA (Cdk13 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Promoter

    U6 Promoter

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR8/GW_TOPO_Cdk13_Untagged

    Plasmid

    #127342

    Purpose

    Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Mutation

    Base pairs 1-1554 of transgene are codon optimize…

    Promoter

    None

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-U6-sgRev-erba-hSyn-mCherry-KASH

    Plasmid

    #223226

    Purpose

    guideRNA targeting the mouse Rev-erb alpha (Nr1d1)

    Depositor

    Kyungjin Kim

    Article

    Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

    Insert

    Nr1d1 (Nr1d1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

    Plasmid

    #217803

    Purpose

    Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Unfused click editor construct; mSA-ePhi29 - pCMV-T7-mSA-Phi29 (JO1411)

    Plasmid

    #217807

    Purpose

    Unfused click editor construct expressing mSA-ePhi29(D169A), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    mSA-linker-SV40NLS-ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Click editor utilizing Cdc13 for template recruitment - pCMV-T7-Cdc13-nCas9-EcKlenow (JO1293)

    Plasmid

    #217796

    Purpose

    Variant CE1 construct with Cdc13 telomere binding protein for template recruitment, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Cdc13-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)

    Plasmid

    #217797

    Purpose

    Variant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Cdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Cdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. shTRIP13-2-seed

    Plasmid

    #184535

    Purpose

    shRNA expression vector

    Depositor

    Andrew Hong

    Article

    Mittal et al Commun Biol. 2024 Apr 8;7(1):426. doi: 1

    Insert

    TRIP13 (TRIP13 Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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