We narrowed to 14,037 results for: Dos

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STAgR_tdTomato

Plasmid

#102993

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Promoter

U6

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_DNAJB1_DNAJ

Plasmid

#109918

Purpose

Protein expression and purification of DNAJB1_DNAJ

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DNAJB1_DNAJ

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-Zfp536

Plasmid

#45845

Depositor

Marius Wernig

Article

Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

Insert

Zfp536 (Zfp536 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet-ON

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pMpGWB120

Plasmid

#68574

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pTpal-fdc

Plasmid

#78288

Purpose

Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

Depositor

David Nielsen

Article

McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

Inserts

PAL2 (PAL2 Mustard Weed)
FDC1 (FDC1 Budding Yeast)

Expression

Bacterial

Mutation

*see below

Promoter

trc

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_SPF45

Plasmid

#110044

Purpose

Protein expression and purification of SPF45

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SPF45 (RBM17 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Actin binding domain of Ezrin

Plasmid

#62381

Purpose

Expresses the actin binding domain of ezrin. Amino acids 552-586.

Depositor

Guillaume Charras

Article

Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

Insert

Actin binding domain of ezrin (EZR Human)

Tags

GFP

Expression

Mammalian

Mutation

contains aa's 552-586

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pSMP-RING1_2

Plasmid

#36356

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

RING1 (RING1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
Sa-dCas9-NLS-3xFLAG/pcDNA3.1

Plasmid

#98041

Purpose

Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

Sa-dCas9-NLS-3xFLAG

Use

CRISPR

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

D10A + H557A

Promoter

CMV

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pOCC304

Plasmid

#118897

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette

Tags

HIS6, cleavable with TEVprotease and TwinStrep-MB…

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV4a-SIRT6-G60A-FLAG

Plasmid

#102323

Purpose

Human SIRT6 G60A with FLAG tag for mammalian expression

Depositor

Hening Lin

Article

Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

Insert

SIRT6 (SIRT6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed glycine 60 to alanine

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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NPY-mApple

Plasmid

#83498

Purpose

Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

Depositor

Sebastian Barg

Article

Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

Insert

NPY (NPY Human)

Tags

mApple

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe-GW-GFP

Plasmid

#40123

Depositor

Lynda Chin

Article

Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

Insert

GFP

Use

Retroviral

Expression

Mammalian

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
untargeted Plk1 FRET sensor Myt1 substrate

Plasmid

#45217

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

untargeted Plk1 FRET sensor Myt1 substrate

Expression

Mammalian

Promoter

t7

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCK015

Plasmid

#105347

Purpose

Bacterial translocation fo proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Mutation

BsaI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pC1-FGB (FlincG2)

Plasmid

#49204

Purpose

Fluorescent reporter for cGMP

Depositor

John Garthwaite

Article

Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

Insert

FlincG2 (PRKG1 GFP, Bovine)

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only

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pCK013

Plasmid

#105345

Purpose

Bacterial translocation fo proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Mutation

BsaI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCK016

Plasmid

#105348

Purpose

Bacterial translocation for proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Mutation

BsaI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCK014

Plasmid

#105346

Purpose

Bacterial translocation fo proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Mutation

BsaI restriction sites removed

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR1H3_Hormone-recep

Plasmid

#109950

Purpose

Protein expression and purification of NR1H3_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR1H3_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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