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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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176,974 results

Showing: 8301 - 8320 of 176974 results

  2. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

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  4. pCRISPR-aBEST

    Plasmid
    #131464
    Purpose
    Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes
    Depositor
    Tilmann Weber
    Article
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Inserts
    Streptomyces codon optimized spCas9n (D10A)
    streptomyces codon optimized tadA
    Use
    CRISPR and Synthetic Biology
    Promoter
    tipA
    Available Since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    131464-plasmid-map-sequence-id-261686

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  7. pCMV-2E2-HA-mEGFP

    Plasmid
    #129595
    Purpose
    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 15, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129595-plasmid-map-sequence-id-254267

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  9. pAAV-CamKII-ArchT-GFP (PV2527) (AAV Retrograde)

    Viral Prep
    #99039-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CamKII-ArchT-GFP (PV2527) (#99039). In addition to the viral particles, you will also receive purified pAAV-CamKII-ArchT-GFP (PV2527) plasmid DNA. CaMKII-driven ArchT-GFP expression for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Han et al Front Syst Neurosci. 2011;5:18. Epub 201
    Promoter
    CaMKII
    Tags
    GFP
    Available Since
    May 15, 2019
    Availability
    Academic Institutions and Nonprofits only

  14. pGEX6P1-DEST

    Plasmid
    #119749
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119749-plasmid-map-sequence-id-233993

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Showing: 8301 - 8320 of 176974 results