164,843 results

1. pJK_kanR_proJK1_PAD

   Plasmid

   #60630

   Purpose

   Constitutively expresses PAD (phenolic acid decarboxylase)

   Depositor

   Timothy Whitehead

   Article

   Tomek et al Biotechnol Bioeng. 2015 Mar;112(3):627-3

   Insert

   Phenolic acid decarboxylase padC (padC )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K146Y

   Promoter

   proJK1

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTHSSe_39

   Plasmid

   #109238

   Purpose

   PTac driving TALEsp1

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   TALEsp1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PTac

   Available since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJSH-B14

   Plasmid

   #20145

   Depositor

   Glen Hartman

   Article

   Haudenshield et al Plant Dis. 2011 Mar;95(3):343-352. doi:

   Insert

   Artificial sequence
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   n/a

   Promoter

   Available since

   Feb. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHIV-INK4A-zsGreen

   Plasmid

   #110728

   Purpose

   Mammalian Expression of p16INK4A IRES zsGreen

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   p16INK4A IRES zsGreen (CDKN2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. YIplac128-pOst1-ESCargo

   Plasmid

   #115421

   Purpose

   Expresses a regulatable secretory cargo for yeast at the LEU2 locus

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

   Insert

   pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TPI1

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB1A3_GFP_dT17

   Plasmid

   #89660

   Purpose

   expression of doubleTAL staple protein dT17 with N-terminally fused cycle3-GFP

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

   Insert

   GFP_dT17
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-YY1

   Plasmid

   #169018

   Purpose

   Expression of YY1 driven by CMV promoter

   Depositor

   Claes Wadelius

   Article

   Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

   Insert

   human YY1 (YY1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmRFP-LC3

   Plasmid

   #21075

   Purpose

   Mammalian expression of rat LC3 fused to mRFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kimura et al Autophagy. 2007;3(5):452-60.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   First ATG is not included.

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. LifeAct-mScarletI pDRF1-GW

   Plasmid

   #168085

   Purpose

   Expresses Lifeact-mScarletI to visualize actin in yeast

   Depositor

   Bas Teusink

   Insert

   LifeAct-mScarletI
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-his-3C_Tau352

    Plasmid

    #100092

    Purpose

    Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

    Depositor

    Nikolai Sluchanko

    Article

    Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

    Insert

    Fetal microtubule-associated protein tau (MAPT Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    wild type sequence

    Promoter

    T7

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSB1A3_GFP_dT21

    Plasmid

    #89662

    Purpose

    expression of doubleTAL staple protein dT21 with N-terminally fused cycle3-GFP

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

    Insert

    GFP_dT21
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSB1A3_GFP_dT32

    Plasmid

    #89667

    Purpose

    expression of doubleTAL staple protein dT32 with N-terminally fused cycle3-GFP

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

    Insert

    GFP_dT32
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSB1A3_GFP_dT16

    Plasmid

    #89659

    Purpose

    expression of doubleTAL staple protein dT16 with N-terminally fused cycle3-GFP

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

    Insert

    GFP_dT16
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. G13-CASE

    Plasmid

    #168127

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at F125/D126 within GNA13

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. BL21 ΔrecBCD

    Bacterial Strain

    #176581

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

    Depositor

    Jean-Loup Faulon, Chase Beisel

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    None

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

16. MiniCoopR 2xU6:gRNA, mitfa:Cas9

    Plasmid

    #118844

    Purpose

    Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Type

    Empty backbone

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCW-eGFP

    Plasmid

    #162823

    Purpose

    Doxycycline-inducible overexpression of eGFP

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    V163A

    Promoter

    Tet ON

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSB1A3_GFP_dT31

    Plasmid

    #89666

    Purpose

    expression of doubleTAL staple protein dT31 with N-terminally fused cycle3-GFP

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

    Insert

    GFP_dT31
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSB1A3_GFP_dT27

    Plasmid

    #89665

    Purpose

    expression of doubleTAL staple protein dT27 with N-terminally fused cycle3-GFP

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

    Insert

    GFP_dT27
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MIGR1

    Plasmid

    #27490

    Depositor

    Warren Pear

    Article

    Pear et al Blood. 1998 Nov 15. 92(10):3780-92.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge