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pPS

Plasmid

#85801

Purpose

Backbone for pooled cloning of Perturb-seq libraries

Depositor

Aviv Regev

Article

Dixit et al Cell. 2016 Dec 15;167(7):1853-1866.e17.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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DHFR-SpCas9-DHFR

Plasmid

#85447

Purpose

Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-SpCas9-DHFR

Use

AAV

Tags

Destabilized domain (C-terminus version) of E.col…

Expression

Mammalian

Promoter

Cbh

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLuc

Plasmid

#83281

Purpose

recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

Firefly Luciferase

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pRCC-N

Plasmid

#81192

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-Zdk1

Plasmid

#81057

Purpose

mammalian expression of Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, and mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TDsmURFP-IRES-eGFP

Plasmid

#80344

Purpose

Expresses Tandem Dimer smURFP and eGFP in mammalian cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

Tandem Dimer smURFP
eGFP

Expression

Mammalian

Promoter

CMV and IRES

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pKA-207I10

Plasmid

#79845

Purpose

Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

NLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR

Expression

Mammalian

Mutation

RpPpsR2 cysteine 439 changed to serine

Promoter

CMV

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP gRNA (BRDN0000563266)

Plasmid

#80036

Purpose

3rd generation lentiviral gRNA plasmid targeting EGFP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3_erGAP3

Plasmid

#78118

Purpose

Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

Depositor

Teresa Alonso, Javier García-Sancho

Article

Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9

Insert

erGAP3

Tags

KDEL and calreticulin signal peptide

Expression

Mammalian

Mutation

S175G, D180Y, I167V and L15Q in GFP moiety and D…

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP Abi1

Plasmid

#74905

Purpose

Expresses human Abi1 fused to GFP

Depositor

Giorgio Scita

Article

Innocenti et al Nat Cell Biol. 2004 Apr;6(4):319-27. Epu

Insert

Abi1 (ABL1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/BFP Dest

Plasmid

#74282

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGM1190

Plasmid

#69994

Purpose

OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Expression

Bacterial

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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SapTrap Kit

Plasmid Kit

#1000000077

Purpose

A modular toolkit for building CRISPR targeting vectors to insert genetic tags in the C. elegans genome.

Depositor

Erik Jorgensen

Application

Genome Editing

Vector Type

Worm Expression

Editing Type

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pLV-tetO-Eomes

Plasmid

#70271

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Eomes

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Eomes (Eomes Mouse)

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ144

Plasmid

#69296

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/SECFP-hATG16A1

Plasmid

#58994

Purpose

Expresses SECFP-hATG16A1 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 16-like 1 (S. cerevisiae) (ATG16L1 Human)

Use

Retroviral

Tags

SECFP

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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GFP_CD47_SU

Plasmid

#65473

Purpose

GFP tagged CD47 with its own short 3'UTR

Depositor

Christine Mayr

Article

Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature

Inserts

Signal peptide of CD47
GFP
CD47 coding region continued and short 3’UTR.

Expression

Mammalian

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pLUT7 HA-GLI1

Plasmid

#62970

Purpose

Expresses HA-tagged Gli1

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

GLI1 (GLI1 Human)

Use

Lentiviral

Tags

HA

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-ZMYND8

Plasmid

#65401

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

ZMYND8 (ZMYND8 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINS-USP21 (USP, aa 196-565)

Plasmid

#61585

Purpose

Expresses human USP21 (USP domain) in E. coli.

Depositor

David Komander

Article

Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

Insert

USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)

Tags

His6-SUMO

Expression

Bacterial

Mutation

Deleted aa 1-195.

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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