180,959 results
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Plasmid#80005PurposeIκBα fused with near-infrared fluorescent protein miRFP703 (IκBα reporter)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIκBα (NFKBIA Human)
TagsmiRFP703ExpressionMammalianPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3_erGAP3
Plasmid#78118PurposeLow affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulumDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserterGAP3
TagsKDEL and calreticulin signal peptideExpressionMammalianMutationS175G, D180Y, I167V and L15Q in GFP moiety and D…PromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEBB HA RelA
Plasmid#74892PurposeExpress RelA in mammalian cellsDepositorAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-mR7BD
Plasmid#79149Purposebacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)DepositorAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP_TRPM8
Plasmid#64879PurposeExpresses Ion Channel TRPM8 fused to eGFP in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTransient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)
TagseGFPAvailable sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGM1190
Plasmid#69994PurposeOriT containing mobilizable vector for thiostrepton induced gene expression in StreptomycesDepositorInsertsaac
tsr
rep
sso
TipA promoter
dso
ExpressionBacterialAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p323-L10A-PATagRFP
Plasmid#74172PurposeFluorescent reporter for single ribosome trackingDepositorAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NLS-mCherry-NES reporter (pDN160)
Plasmid#72660PurposeNLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-mCherry-NES
ExpressionMammalianPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a SnoopTag-mEGFP-SpyTag
Plasmid#72325PurposeBacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.DepositorInsertpET28a SnoopTag-mEGFP-SpyTag
TagsN-terminal His6 tagExpressionBacterialAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pST44
Plasmid#64007Purposepolycistronic cloning vector for pST44 plasmid suiteDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ144
Plasmid#69296PurposeGolden Gate recipient and Gateway entry vector; assembly of 4 gRNAsDepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT040
Plasmid#67640PurposeURA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassetteDepositorInsertsingle guide RNA expression cassette
UseCRISPRExpressionYeastPromoterpSNR52Available sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5FRT-EF-Pdgfrbeta-EGFPN
Plasmid#66790PurposeExpression of murine PDGFRbeta tagged with GFP under the control of EF1a promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPDGFRbeta (Pdgfrb Mouse)
TagsC-term EGFPExpressionMammalianPromoterEF1aAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA
Plasmid#65455PurposeCan be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinasesDepositorInsertEGFP
UseAAVPromoterCAGAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP_CD47_SU
Plasmid#65473PurposeGFP tagged CD47 with its own short 3'UTRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsSignal peptide of CD47
GFP
CD47 coding region continued and short 3’UTR.
ExpressionMammalianAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBa-MARK2-eGFP
Plasmid#66706Purposemammalian expression vector for MARK2 with a C-terminal eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMARK2 (MARK2 Human)
TagseGFPExpressionMammalianPromoterchicken beta actinAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-KDEL_neomycin
Plasmid#65306PurposeLuminal ER hook only (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStreptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
TagsKDEL motif and Signal SequenceExpressionMammalianPromoterCMVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CRISPR-SP-Cas9 reporter
Plasmid#62733PurposeMammalian CRISPR-SP-Cas9 reporterDepositorInsertCRISPR-SP-Cas9 target seq + tdTomato (out of frame)
UseCRISPR and LentiviralTagsCRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…ExpressionMammalianPromoterEF1-AlphaAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSIN4-EF1a-GATA2-IRES-Puro
Plasmid#61063Purposelentiviral vector for GATA2DepositorAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA-eGFP-Reporter
Plasmid#60719PurposeExpresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA (5'-AAAGGTCGAGAAACTGCAAA-3')
UseCRISPRExpressionMammalianPromoterU6 and mouse U6Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by