167,785 results

1. miCBE

   Plasmid

   #205413

   Purpose

   Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with APOBEC3A and UGI driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pU6__RNA*_pCAG_bpNLS_APOBEC3A(W104A)_XTEN_OgeuIscB*(D61A)_NLS_2xUGI_bGHployA_pCMV_mCherry
   

   Expression

   Mammalian

   Mutation

   D61A+E85R+H369R+S387R+S457R

   Promoter

   hU6, CAG, CMV

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. OTUB1

   Plasmid

   #25149

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   otub1.040.271 (OTUB1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   amino acid residues 40-271

   Available Since

   Aug. 6, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G

   Plasmid

   #194458

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-CD19 CAR
   

   Use

   Lentiviral

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. hiActTS-GR

   Plasmid

   #79775

   Purpose

   high FRET control for ActTS-GR

   Depositor

   Tetsuya Kitaguchi

   Article

   Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

   Insert

   hiActTS-GR
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-MEF2C-HA

   Plasmid

   #32515

   Depositor

   Andrew Lassar

   Article

   Kozhemyakina et al Mol Cell Biol. 2009 Nov;29(21):5751-62.

   Insert

   Mef2c (Mef2c Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Sox2-CreERT2

   Plasmid

   #135618

   Purpose

   Donor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locus

   Depositor

   Lin He

   Article

   Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

   Insert

   P2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   P2A-CreERT2

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 iCasper T2A HO1

   Plasmid

   #64278

   Purpose

   Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

   Depositor

   Xiaokun Shu

   Article

   To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Insert

   Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEmerald-Fascin-C-10

   Plasmid

   #54094

   Purpose

   Localization: Actin Bundling Protein, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Fascin (FSCN1 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. Human Metabolic Gene CRISPRa sgRNA library

   Pooled Library

   #187080

   Purpose

   Lentiviral CRISPRa library targeting human metabolic genes for activation of gene expression

   Depositor

   Kivanc Birsoy

   Article

   Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   Lentiviral

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

10. Cultivarium MACKEREL Library

    Pooled Library

    #208054

    Purpose

    pGL0_151 [RS421] is a library of broad host range prokaryotic promoters-RBS regulatory sequences formatted as a golden gate assembly Level 0 part.

    Depositor

    Cultivarium Tools, Charlie Gilbert, Nili Ostrov

    Article

    Gilbert et al bioRxiv 2024.02.23.581749

    Use

    Synthetic Biology

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

11. PKC epsilon WT

    Plasmid

    #21240

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC epsilon (Prkce Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1 TEV (full-length)

    Plasmid

    #64276

    Purpose

    Expresses tobacco etch virus protease (TEV)

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Tobacco etch virus (TEV) protease
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV9)

    Viral Prep

    #87306-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Promoter

    EF1a

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

14. pKM201

    Plasmid

    #13078

    Depositor

    Kenan Murphy

    Article

    Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

    Insert

    Ptac-gam-bet-exo operon
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB CAG-sfGFP-T2A-iCreERT2

    Plasmid

    #177309

    Purpose

    CAG-sfGFP-T2A-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP-T2A-iCreERT2
    

    Use

    Cre/Lox; Piggybac

    Expression

    Mammalian

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EFS-SpCas9

    Plasmid

    #104588

    Purpose

    AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    Streptococcus pyogenes Cas9
    

    Use

    AAV and CRISPR

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.TBG.PI.Null.bGH

    Plasmid

    #105536

    Purpose

    empty AAV vector with TBG promoter

    Depositor

    James M. Wilson

    Has Service

    AAV8

    Insert

    None
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TBG

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. TP53-KO_gRNA_1

    Plasmid

    #195130

    Purpose

    TP53-targeting gRNA in the LRG2.1 backbone

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    TP53 KO gRNA 1 (TP53 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-SIRT2-Flag

    Plasmid

    #102624

    Purpose

    Expresses full length SIRT2 isoform 1 in mammalian cells

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    SIRT2 (SIRT2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-Beta3

    Plasmid

    #140988

    Purpose

    Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GBeta3 (GNB3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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