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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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180,111 results

Showing: 8261 - 8280 of 180111 results

  1. Flag-M4-AR

    Plasmid
    #171240
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor (hAR)
    Depositor
    Steven Balk
    Publication
    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFlag-tagged human androgen receptor (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    171240-plasmid-map-sequence-id-336015

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  2. pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet

    Plasmid
    #162280
    Purpose
    Eukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Publication
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SCN8A Variant 1, stabilized (SCN8A Human)
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    Modified human HSPA5 intron 4 inserted after bp23…
    Promoter
    CMV
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    162280-plasmid-map-sequence-id-334621

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  3. pAAV-nEF-Con/Fon-ChR2-mCherry (AAV8)

    Viral prep
    #137142-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-ChR2-mCherry (#137142). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-ChR2-mCherry plasmid DNA. nEF-driven, Cre and Flp-dependent expression of channelrhodopsin fused to mCherry for optogenetic activation.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    mCherry (Cre and Flp-dependent)
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only

  4. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV9)

    Viral prep
    #87306-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Li Zhang
    Publication
    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.
    Promoter
    EF1a
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  6. pC1-HyPerRed-mito

    Plasmid
    #60247
    Purpose
    Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.
    Depositor
    Vsevolod Belousov
    Publication
    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyPerRed-mito
    Tags
    Mitochondrial targeting signal
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    60247-plasmid-map-sequence-id-317302

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  8. pAAV-hSyn-Cre-P2A-dTomato (AAV8)

    Viral prep
    #107738-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Rylan Larsen
    Promoter
    Syn
    Tags
    dTomato (physically separate, not a fusion protein)
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only

  9. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid
    #159107
    Purpose
    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).
    Depositor
    Thomas Tuschl
    Publication
    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.
    Insert
    NSP12
    Tags
    His-SUMO tag
    Expression
    Bacterial
    Mutation
    None
    Promoter
    T7
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    159107-plasmid-map-sequence-id-310025

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  14. pCDH-EF1-IRES eGFP

    Plasmid
    #128059
    Purpose
    Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    128059-plasmid-map-sequence-id-256675

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  16. pCMV-2E2-HA-mEGFP

    Plasmid
    #129595
    Purpose
    Encodes anti-HA frankenbody variant fused with mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Publication
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129595-plasmid-map-sequence-id-254267

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Showing: 8261 - 8280 of 180111 results