171,537 results
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Plasmid#126703PurposeConstitutive, ubiquitous mCherry expression.DepositorInsertmCherry
UseLentiviralPromoterEf1aLAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2545
Plasmid#144038PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT13-splitC
Plasmid#122596Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
ExpressionBacterialAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXsIP GFP-VMP1
Plasmid#117417PurposeExpresses VMP1 tagged with GFP in mammalian cellsDepositorAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
IRES-creERT2-FNF
Plasmid#188230PurposeEMCV IRES-cre-ERT2-FRT-Neo*-FRT cassetteDepositorInsertIRES-creERT2-FNF
UseCre/Lox and Mouse TargetingExpressionBacterial and MammalianAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR U6-hTERTsh
Plasmid#111385PurposeKnock down of hTERT transcriptDepositorInsertshRNA C3 against hTERT
UseGateway entry cloningPromoterU6Available SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-P2A-EGFP (RTW4830)
Plasmid#139989PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP30
Plasmid#22578DepositorAvailable SinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
(383) pcDNA3.1-HA-nBC2-OGA(544-706)
Plasmid#168100PurposeHA- tagged BC2 nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGADepositorInsertHA-nBC2-OGA(544-706)
TagsHA-tagExpressionMammalianMutationOGA truncated to contain residues 544-706PromoterT7/CMVAvailable SinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV/3' Box_(GLuc)_TOP1
Plasmid#68434PurposeTransient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.DepositorInsertTOP1 construct
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pL
Plasmid#196286Purpose35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)DepositorInsertFull length TSWV L antigenome encoding codon-optimized L (RdRp)
UseCRISPRExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ionRD
Plasmid#172931PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.DepositorInsertIonicStrengthSensorRD
ExpressionMammalianPromoterCMVAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.10
Plasmid#102919Purposeexpresses ABE7.10 in mammalian cellsDepositorInsertABE7.10
TagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo
Plasmid#183411PurposepiggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.DepositorInsertTet-on 3G
UseCRISPR and Synthetic Biology; PiggybacExpressionMammalianPromoterEF1AAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-Ataxin3Q84
Plasmid#22124DepositorAvailable SinceOct. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV
Plasmid#235534PurposeTo express GFPDepositorTypeEmpty backboneUseLentiviralAvailable SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER- PM Long P2A
Plasmid#164109PurposeDetect the long Plasma membrane--mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP and YFP PM-Mt-ER Long P2A
ExpressionMammalianAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
CIDAR MoClo Parts Kit
Plasmid Kit#1000000059PurposeCollection of standardized parts for combinatorial design and expression tuning in E. coli.DepositorApplicationCloning and Synthetic BiologyVector TypeBacterial ExpressionCloning TypeGolden Gate (MoClo)Available SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX_lenti_osTir1_9Myc_P2A_Bsd
Plasmid#139746Purposelentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection markerDepositorInsertrice E3 ligase Tir1 (osTir1)
ExpressionMammalianPromoterEF1alphaAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Kv1.2 K+ channel scFv [K14/16]
Plasmid#182064PurposeMammalian Expression of Kv1.2 K+ channel scFv. Derived from hybridoma K14/16.DepositorInsertrecombinant mouse scFv targeting Kv1.2 K+ channel (Homo sapiens) (KCNA2 Mouse)
TagsHA, HisExpressionMammalianPromoterCMVAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only