We narrowed to 15,187 results for: pET

1. pZac2.1-GfaABC1D-GAT3-HA-mCherry

   Plasmid

   #117722

   Purpose

   Expresses a GABA transporter GAT-3 (Slc6a11)

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Insert

   Slc6a11 (Slc6a11 Mouse)
   

   Use

   AAV

   Tags

   HA and mCherry

   Expression

   Mammalian

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX-evoCas9

   Plasmid

   #107550

   Purpose

   Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

   Depositor

   Anna Cereseto

   Article

   Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

   Insert

   Humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   M495V/Y515N/K526E/R661Q

   Promoter

   CBh

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHL 1405

   Plasmid

   #53010

   Purpose

   pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   RhyB sRNA
   DsrA sRNA
   hfq
   sodB
   

   Use

   Synthetic Biology

   Tags

   gfp

   Expression

   Bacterial

   Mutation

   contains -56 to +141 relative to start codon

   Promoter

   pCon, pLlacO-1, and pLtetO-1

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCA528-PPxY1-GS-WW3e

   Plasmid

   #166705

   Purpose

   expresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   AMOT PPxY1 fused to NEDD4L WW3
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. siRNA-resistant SigmaR1-mNeonGreen R119A

   Plasmid

   #226572

   Purpose

   Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   R119A

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. siRNA-resistant SigmaR1-mNeonGreen D188A

   Plasmid

   #226573

   Purpose

   Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   D188A

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. siRNA-resistant SigmaR1-mNeonGreen F191A

   Plasmid

   #226571

   Purpose

   Encodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   F191A

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTO149_RFP_CauNEO

   Plasmid

   #177277

   Purpose

   Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.

   Depositor

   Teresa O'Meara

   Article

   Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

   Insert

   RFP, CauNEO
   

   Tags

   ENO1-RFP

   Expression

   Bacterial

   Mutation

   All CUG Leucine codons were replaced with the syn…

   Promoter

   AgTEF2

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330_DRS-1 sgRNA / hSpCas9

   Plasmid

   #172841

   Purpose

   Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-S in pAR9 (rep/cap)

    Plasmid

    #174539

    Purpose

    encodes AAV-S capsid for efficient gene delivery to the mammalian cochlea

    Depositor

    Casey Maguire

    Article

    Ivanchenko et al Mol Ther Methods Clin Dev. 2021 Mar 29;2

    Insert

    VP1 of AAV9 containing the AAV-S 7-mer peptide insert
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCB036 FoMV-sgGFP

    Plasmid

    #234813

    Purpose

    To express gRNA from Foxtail Mosaic Virus Vector targeting GFP (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    gRNA targeting GFP
    

    Expression

    Plant

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSP1673

    Plasmid

    #65769

    Purpose

    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMVHA E2F2

    Plasmid

    #24226

    Purpose

    Mammalian expression of human E2F2 with HA tag

    Depositor

    Kristian Helin

    Article

    Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

    Insert

    E2F2 (E2F2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSMART-sgRNA (Sp)

    Plasmid

    #80427

    Purpose

    U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO mouse shRNA 1 rictor

    Plasmid

    #21341

    Depositor

    David Sabatini

    Article

    Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

    Insert

    rictor shRNA 1 (Rictor Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pR26 CAG/BFP Dest

    Plasmid

    #74282

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57-Amp_CRISPIE donor B1

    Plasmid

    #172842

    Purpose

    CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-0) encoding mEGFP
    

    Use

    CRISPR

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. MMLV-RT(dRH) for Split-PE (pEH50)

    Plasmid

    #190106

    Purpose

    Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    MMLV-RT(delta RNAseH)
    

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    mutations from RT in PE2 and truncation of RNAse …

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. gfp-Arkadia/RNF111

    Plasmid

    #112228

    Purpose

    Expresses GFP-tagged Arkadia in mammalian cells

    Depositor

    Vasso Episkopou

    Article

    Mavrakis et al PLoS Biol. 2007 Mar;5(3):e67. doi: 10.13

    Insert

    (Arkadia)Rnf111 (Rnf111 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-CAG-ECC-Biosensor

    Plasmid

    #140832

    Purpose

    Encoding the CAG promoter based eccDNA biosensor

    Depositor

    Yonglun Luo

    Article

    Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

    Insert

    ECC-Biosensor
    

    Use

    Piggybac (pb) transposon

    Expression

    Mammalian

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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