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pRG710_lexO-HO_LexA-TF_HIS3MX

Plasmid

#154819

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Publication

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Tags

FLAG

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJAS1668

Plasmid

#158593

Purpose

VioE-ePTS1 CEN6/ARS4

Depositor

John Dueber

Publication

Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

Insert

VioE-ePTS1

Expression

Yeast

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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nuc-yHS1-M7A,H102A

Plasmid

#159168

Purpose

Nuclear labile heme reporter

Depositor

Amit Reddi

Publication

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

nuc-yHS1-M7A,H102A

Tags

SV40 NLS

Expression

Yeast

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

GPD

Available since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBS1479-HTP-KanMX6

Plasmid

#154085

Purpose

For C-terminal his6-TEV-ProtA-tagging in S. pombe using KanMX6 as resistance marker.

Depositor

Lidia Vasiljeva

Publication

Kilchert et al Cell Rep. 2015 Dec 22;13(11):2504-2515.

Insert

his6-TEV-protein A-KanR

Use

Pcr-based tagging in yeast

Tags

his6-TEV-protein A

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBKWH-Lam

Plasmid

#133899

Purpose

Negative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Publication

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

LMNA (Lam) (LMNA Human)

Expression

Yeast

Promoter

T7

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEM09

Plasmid

#135479

Purpose

Spizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFP

Depositor

Nicolas Buchler

Publication

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunHsp70pr

Tags

GFP and hph

Expression

Bacterial and Yeast

Promoter

Spun Hsp70pr

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGWKS2

Plasmid

#139410

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised GFP S65T marker.

Depositor

James Fraser

Publication

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

GFP S65T

Expression

Yeast

Promoter

TEF1

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPICZ:mTREK-1_cryst

Plasmid

#133269

Purpose

P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP

Depositor

Dan Minor

Publication

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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TEF2pro_mCherry_YFP

Plasmid

#138623

Purpose

Dual reporter backbone plasmid for CCRA experiment

Depositor

Rob Mitra

Type

Empty backbone

Expression

Yeast

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMS22H

Plasmid

#133904

Purpose

Expression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Publication

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Expression

Yeast

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRS313-PRP8

Plasmid

#89966

Purpose

complements prp8 genomic deletion

Depositor

Christine Guthrie

Publication

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

none

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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BRR2 pRS313

Plasmid

#111411

Purpose

Brr2 (endogenous promoter)

Depositor

Christine Guthrie

Publication

Mayerle et al bioRxiv 2018. 354514

Insert

BRR2

Expression

Yeast

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pGAN101

Plasmid

#131123

Purpose

Plasmid for overexpression of prion domains to induce prions

Depositor

Ahmad Khalil

Publication

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Tags

mKate2

Expression

Yeast

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSK226

Plasmid

#129268

Purpose

dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter

Depositor

Ahmad Khalil

Publication

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Rnq1

Expression

Yeast

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCUP1pNuiHA kanMX CEN

Plasmid

#131168

Purpose

for ScCUP1 promoter regulated N-terminal fused Nui-HA prey expression, centromeric ARS plasmid, kanMX marker confers resistance to geneticin in S. cerevisiae

Depositor

Nils Johnsson

Publication

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Tags

Nui-HA

Expression

Yeast

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMJA104

Plasmid

#128520

Purpose

Expresses amyloid human Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Publication

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme I56T (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA065

Plasmid

#128517

Purpose

Expresses Ber e 1 in Pichia

Depositor

Marcos Alcocer

Publication

Alcocer et al J Mol Biol. 2002 Nov 15;324(1):165-75.

Insert

Ber e 1 a allergenic 2S albumin from Brazil nut

Expression

Yeast

Promoter

AOX1

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

Plasmid

#124278

Purpose

Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

Depositor

Arthur Laganowsky

Publication

Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

Insert

GIRK2 (Kcnj6 Mouse)

Tags

eGFP-StreptagII-6xHis

Expression

Yeast

Mutation

R201A

Promoter

AOX1

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pWS033

Plasmid

#123056

Purpose

Green fluorescent protein

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

sfGFP [3]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRC063

Plasmid

#123057

Purpose

Blue fluorescent protein

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

mTagBFP2 [3]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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