We narrowed to 8,835 results for: sgRNA

1. pKLV-gRNA-Bcan-Ntrk1_4

   Plasmid

   #136413

   Purpose

   Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Insert

   U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV-gRNA-Myb-Qk_1

   Plasmid

   #136415

   Purpose

   Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Insert

   U6_sgRNA(Myb)_U6_sgRNA(Qk)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Adeno BN

   Plasmid

   #101823

   Purpose

   Adenovirus for the expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1

   Depositor

   Andrea Ventura

   Article

   Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10

   Insert

   U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)_CBh_FLAG-Cas9 (Bcan, Ntrk1 Mouse)
   

   Use

   Adenoviral

   Tags

   FLAG-Cas9

   Available Since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

4. dTAG-IRF8

   Plasmid

   #190620

   Purpose

   Lentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradation

   Depositor

   Junwei Shi

   Article

   Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

   Insert

   IRF8 (IRF8 Human)
   

   Use

   Lentiviral

   Tags

   FKBP12_G36V

   Expression

   Mammalian

   Mutation

   synonymous mutation for sgRNA resistance for sgIR…

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL21-VEX

   Plasmid

   #158230

   Purpose

   Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)

   Depositor

   Junwei Shi

   Article

   Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Promoter

   U6 promoter for crRNA expression and EFS promoter…

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

   Plasmid

   #156432

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

   Plasmid

   #73796

   Purpose

   pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

   Depositor

   Ronald Davis

   Article

   Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

   Inserts

   dCas9-Mxi1
   Tet Repressor
   Structural gRNA for S pyogenes
   

   Use

   CRISPR

   Tags

   Mxi1 and NLS

   Expression

   Yeast

   Mutation

   D10A, H840A

   Promoter

   pGPM1, pRPR1(TetO), and pTef1

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPRv2-sgCTL

   Plasmid

   #234771

   Purpose

   Non Targeting Control

   Depositor

   Judy Lieberman

   Article

   Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

   Insert

   Non Targeting Control sgRNA
   

   Use

   Lentiviral

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGH020 sgp53

   Plasmid

   #227947

   Purpose

   sgRNA targeting p53, GFP, neomycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

   Depositor

   Laura Attardi

   Article

   Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

   Insert

   Tp53 (Trp53 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CRISPRv2-sgIl1r1_#2-hygro

    Plasmid

    #231990

    Purpose

    Knockout mouse Il1r1

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 and hygromycin resistance (Il1r1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX458-3xHA-en31FnCas9

    Plasmid

    #201949

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en31FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    G1243T, E1369R, E1603H on FnCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSL21-mCherry

    Plasmid

    #164410

    Purpose

    Retroviral expression plasmid of sgRNA with mCherry

    Depositor

    Junwei Shi

    Article

    Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

    Plasmid

    #209196

    Purpose

    Mutagenesis of Cnr1

    Depositor

    Larry Zweifel

    Article

    Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

    Insert

    Cnr1 (Cnr1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Promoter

    CMV

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-sgCrebbp#2/Cre

    Plasmid

    #193210

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgCrebbp#2 (Crebbp Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgAPC(B)

    Plasmid

    #85558

    Purpose

    Expresses sgRNA targeting human APC around codon I1417

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    APC, WNT signaling pathway regulator (APC Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid

    #156429

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Mutation

    DELTA1-265

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX458-3xHA-en1FnCas9

    Plasmid

    #201947

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en1FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    E1369R on FnCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #223321

    Purpose

    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.

    Depositor

    Jonathan Arias

    Article

    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

    Inserts

    SpCas9 codon optimized for human (cas9 Human, Synthetic)
    chloramphenicol resistance protein
    CcdB
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid

    #163643

    Purpose

    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    

    Use

    Lentiviral

    Tags

    Luc2-P2A-tdTomato-T2A

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    EFS and pU6

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid

    #156434

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Mutation

    All zinc fingers mutated

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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