We narrowed to 8,835 results for: sgRNA

1. lentiCRISPR v2 puro - CRBN (#1)

   Plasmid

   #166240

   Purpose

   sgRNA (#1) to generate a knockout of human CRBN by targeting the middle region of Exon1

   Depositor

   Günter Schneider

   Article

   Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

   Insert

   CRBN (CRBN Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458-sgEIF4EBP1

   Plasmid

   #128097

   Purpose

   CRISPR gRNA against human EIF4EBP1 (4EBP1) with Cas9 from S. pyogenes and 2A-EGFP

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   CRISPR sgRNA against human 4EBP1 (EIF4EBP1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-sgCrebbp#1/Cre

   Plasmid

   #193209

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgCrebbp#1 (Crebbp Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDECKO_GFP

   Plasmid

   #141210

   Purpose

   empty GFP vector for cloning sgRNA or pgRNAs

   Depositor

   Rory Johnson

   Article

   Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdCas9-DNMT3A-PuroR (ANV)_v2

   Plasmid

   #74408

   Purpose

   Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E756A inactiva…

   Promoter

   CBh

   Available Since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR v2 puro - CRBN (#2)

   Plasmid

   #166241

   Purpose

   sgRNA (#2) to generate a knockout of human CRBN by targeting the start region of Exon1

   Depositor

   Günter Schneider

   Article

   Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

   Insert

   CRBN (CRBN Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAU003 - pTRE3G-Cterm_Nterm_switch_CTCF-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

   Plasmid

   #156430

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Mutation

   Cterm_Nterm_switched

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458-3xHA-en15FnCas9

   Plasmid

   #201948

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en15FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   E1603H on FnCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75240

   Purpose

   CRISPR/Cas9 plasmid against human Ikaros

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Ikaros (IKZF1 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. U6-sgR26-eCas9-T2A-BlastR

    Plasmid

    #172493

    Purpose

    For CRISPR-assisted HDR, Rosa26 locus

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Insert

    eCas9, BlastR and sgRNA targeting the Rosa26 (R26) safe harbor locus
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75241

    Purpose

    CRISPR/Cas9 plasmid against human Ikaros

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75243

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75242

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXPR_BRD051d_sgCiPELO #2

    Plasmid

    #229018

    Purpose

    Expression of CRISPRi PELO sgRNA #2

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    PELO (PELO Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXPR_BRD051d_sgCiPELO #1

    Plasmid

    #229017

    Purpose

    Expression of CRISPRi PELO sgRNA #1

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    PELO (PELO Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX458-3xHA-FnCas9ABEmax8.17d

    Plasmid

    #201955

    Purpose

    Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D11A on FnCas9, V82S, V106W, Q154R on mutant TadA

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-FLEX-SaCas9-U6-sgKcnma1

    Plasmid

    #209199

    Purpose

    Mutagenesis of Kcnma1

    Depositor

    Larry Zweifel

    Article

    Juarez et al Sci Adv. 2023 Aug 11;9(32):eadg8869. doi

    Insert

    Kcnma1 (Kcnma1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Promoter

    CMV

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXS-IRES-BLAST flag-CAD 3E@3R

    Plasmid

    #188149

    Purpose

    Expresses C-terminal flag-tagged human CAD 3E@3R in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXS-IRES-BLAST flag-CAD R1398A A1400E R1403A R1404A L1405A

    Plasmid

    #188138

    Purpose

    Expresses C-terminal flag-tagged human CAD R1398A A1400E R1403A R1404A L1405A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-sgBrip1#2/Cre

    Plasmid

    #193204

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Brip1 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgBrip1#2 (Brip1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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