We narrowed to 3,278 results for: multiplex

1. pCF.111

   Plasmid

   #227187

   Purpose

   pORTMAGE-Ec1 + supplement E. coli SSB

   Depositor

   Seth Shipman

   Article

   Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

   Insert

   CspRecT, mutL_E32K, E. coli SSB
   

   Expression

   Bacterial

   Mutation

   mutL E32K

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ150

   Plasmid

   #69301

   Purpose

   Gateway entry vector with pcoCas9 (Plant codon optimized)

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pcoCas9 (plant codon-optimized)
   

   Use

   CRISPR

   Tags

   3XFLAG

   Mutation

   NLS fusion

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGLuc

   Plasmid

   #135288

   Purpose

   GBPart - GLuc Luciferase CDS

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   Gaussia Luciferase
   

   Use

   Luciferase and Synthetic Biology

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Salsa-BC1-P1A1

   Plasmid

   #202842

   Purpose

   Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1

   Depositor

   Noah Palm

   Article

   Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

   Insert

   EGFP/BC1
   

   Expression

   Mammalian

   Promoter

   Tet Response Element (TRE)

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRU294

   Plasmid

   #167689

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::Cas9i
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSF278

   Plasmid

   #167673

   Purpose

   Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

   Plasmid

   #163056

   Purpose

   mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   mito-roGFP2-Grx1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CT438

   Plasmid

   #233666

   Purpose

   Red light-inducible Flp-N; Flp-N27-PhyA

   Depositor

   Wilson Wong

   Article

   Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

   Insert

   Flp-N27-PhyA
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CAG

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. CT447

   Plasmid

   #233663

   Purpose

   Red light-inducible Flp-C; Flp-28C-FHY1

   Depositor

   Wilson Wong

   Article

   Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

   Insert

   Flp-28C-FHY1
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CAG

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCyCBE

    Plasmid

    #226505

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

    Depositor

    Weizhong Chen

    Article

    Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

    Inserts

    APOBEC1-Cas9D10A
    sgRNA
    sacB gene
    

    Use

    CRISPR

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSP3545-dCas9Str

    Plasmid

    #153516

    Purpose

    Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    dCas9Str
    

    Expression

    Bacterial

    Promoter

    nisA

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRU55

    Plasmid

    #167684

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::asCpf1
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

    Plasmid

    #175521

    Purpose

    Nuclear expression of HaloTag10 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag10
    

    Expression

    Mammalian

    Mutation

    HaloTag10 = HaloTag7-Q165W

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCCl-MCS_miniCMV_EGFP

    Plasmid

    #134984

    Purpose

    The plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    EGFP
    

    Use

    Lentiviral

    Promoter

    minCMV

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCE059-SiT-Cas12a-[Activ]

    Plasmid

    #128136

    Purpose

    Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

    Depositor

    Randall Platt

    Article

    Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

    Insert

    AsCas12a-[Activ]-Triplex
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330A_D10A-1x2

    Plasmid

    #58772

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

    Plasmid

    #175522

    Purpose

    Nuclear expression of HaloTag11 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag11
    

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. act5C-mScarlet

    Plasmid

    #230906

    Purpose

    Ubiquitous expression of mScarlet in Drosophila melanogaster

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    mScarlet
    

    Expression

    Insect

    Promoter

    act5C

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb

    Plasmid

    #175544

    Purpose

    Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    IgKchL_HA-HaloTag9-myc_PDGFRtmb
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV hUbC-dCas9-T2A-GFP

    Plasmid

    #53191

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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