We narrowed to 13,002 results for: LIC

1. pMSCVneo-DEST

   Plasmid

   #119746

   Purpose

   Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Harley et al Nat Genet. 2016 Jan;48(1):36-43. doi: 10

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET42b-BE3

   Plasmid

   #87437

   Purpose

   Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

   Insert

   BE3 (Apobec1 Bacillus phage PBS2, Human, Rat)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHIV-BRAFV600E-mOrange2

   Plasmid

   #110732

   Purpose

   Mammalian Expression of BRAFV600E IRES H2B-mOrange2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   BRAFV600E IRES H2B-mOrange2 (BRAF Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   BRAF V600E

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV_NESPylRS(AF)_hU6tRNAPyl

   Plasmid

   #223508

   Purpose

   PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

   Inserts

   NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y306A; Y384F

   Promoter

   CVM, SP6 and U6

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNIC-ZB-SPRTNwt-FL

   Plasmid

   #110217

   Purpose

   Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

   Depositor

   Kristijan Ramadan

   Article

   Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   P296L (see depositor comments below)

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pExp-xMBP-ACE2-CHis

   Plasmid

   #194998

   Purpose

   Production of ACE2 extracellular domain

   Depositor

   Marko Hyvönen

   Article

   Harman et al J Med Chem. 2023 Jul 27;66(14):9881-9893

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Tags

   His8 and xMBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTriEx-mCerulean-PA-Rac1

   Plasmid

   #22030

   Purpose

   Expression of photoactivatable Rac1

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1 (RAC1 Avena sativa (oat), Human)
   

   Use

   Tags

   6X His and mCerulean

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Rac1 starts at I4 and contains mutations Q61L, E9…

   Promoter

   CMV, p10

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTriEx-mCherry-PA-Rac1-C450A

   Plasmid

   #22028

   Purpose

   Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
   

   Use

   Tags

   6xHis and mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…

   Promoter

   CMV, p10

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-M-Flag-BAP1-C91A

   Plasmid

   #125841

   Purpose

   express mutant BAP1-C91A

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Insert

   BAP1 (BRCA1 associated protein 1) (BAP1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   C91A

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriEx-mCherry-PA-Rac1-T17N

    Plasmid

    #22029

    Purpose

    Expression of photoactivatable Rac1 (dominant-negative mutant).

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6xHis and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCX-DI-2A

    Plasmid

    #74671

    Purpose

    Mammalian expression of DI-2A (hCD73/F2A/hCD39)

    Depositor

    Roberto Giovannoni

    Article

    De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

    Inserts

    CD73 (NT5E Human)
    CD39 (ENTPD1 Human)
    

    Use

    Tags

    F2A

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 Mfn2-HA S27A

    Plasmid

    #139193

    Purpose

    Expresses C-terminal HA tagged human Mfn2 S27A in mammalian cells

    Depositor

    Allan Weissman

    Article

    Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

    Insert

    MFN2 (MFN2 Human)
    

    Use

    Tags

    3HA

    Expression

    Mammalian

    Mutation

    Serine 27 to Alanine

    Promoter

    CMV promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-hGPR56-IRES-GFP

    Plasmid

    #52297

    Purpose

    Expresses human GPR56 and GFP in mammalian cells.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    Human GPR56 coding sequence (ADGRG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 Mfn2-HA S27D

    Plasmid

    #139194

    Purpose

    Expresses C-terminal HA tagged human Mfn2 S27D in mammalian cells

    Depositor

    Allan Weissman

    Article

    Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

    Insert

    MFN2 (MFN2 Human)
    

    Use

    Tags

    2HA

    Expression

    Mammalian

    Mutation

    Serine 27 to Aspartic Acid

    Promoter

    CMV promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_RHEB_WT

    Plasmid

    #81257

    Purpose

    Gateway Donor vector containing RHEB , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RHEB (RHEB Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFUSEss-CHIg-mG3_M18

    Plasmid

    #82356

    Purpose

    Expression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

    Insert

    mouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    hEF1-HTLV prom

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV HA hRb delta CDK

    Plasmid

    #58906

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    all 15 Ser/Thr Cdk acceptor sites changed to Ala,…

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe-Puro-IKBalpha-mut (super repressor)

    Plasmid

    #15291

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    inhibitor of Kappa B alpha (NFKBIA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S32A/S36A: “super-repressor” allele (resistant to…

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. Kif1bβ-pcDNA3

    Plasmid

    #22534

    Depositor

    William Kaelin

    Article

    Schlisio et al Genes Dev. 2008 Apr 1. 22(7):884-93.

    Insert

    Kif1bβ (KIF1B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBabe-GFP-IKBalpha-mut (super repressor)

    Plasmid

    #15264

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKB alpha (NFKBIA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Serine 32 to Alanine, Serine 36 to Alanine

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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