Self-amplifying RNA enables rapid, durable, integration-free programming of hiPSCs.
Della Santina CM, Ploessl DS, Lindsay-Mosher N, Brown CG, Ehmann ME, Blanch-Asensio A, Chang Kim E, Boyden ES, Galloway KE, Boyer LA
Cell Rep Methods. 2026 Sep 16:101602. doi: 10.1016/j.crmeth.2026.101602.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 246033 | pKG03372-VEE-eGFP-IRES-PuroR-polyA | IVT template for making eGFP-IRES-PuroR saRNA; polyA encoded on template |
| 246034 | pKG04056-VEE-eGFP-IRES-BleoR-polyA | IVT template for making eGFP-IRES-PuroR saRNA; polyA encoded on template |
| 246035 | pKG03865-VEE-jRCaMP1b-IRES-PuroR-polyA | IVT template for making jRCaMP1b-IRES-PuroR saRNA; polyA encoded on template |
| 246036 | pKG03866-VEE-ASAP5-IRES-PuroR-polyA | IVT template for making ASAP5-IRES-PuroR saRNA; polyA encoded on template |
| 246037 | pKG04057-VEE-HyPer7-IRES-PuroR-polyA | IVT template for making HyPer7-IRES-PuroR saRNA; polyA encoded on template |
| 246038 | pKG03863-VEE-Ngn2-P2A-eGFP-IRES-PuroR-polyA | IVT template for making Ngn2-P2A-eGFP-IRES-PuroR saRNA; polyA encoded on template |
| 246040 | pKG04063-pSHIP-CAG.d-eGFP-IRES-PuroR-bGH | Mammalian expression vector for eGFP-IRES-PuroR transgene |
| 246041 | pKG03869-pIVT-eGFP-IRES-PuroR-polyA | IVT template for making eGFP-IRES-PuroR modRNA; polyA encoded on template |
| 246057 | pKG04347-VEE-GINKO1-IRES-PuroR-polyA | IVT template for making GINKO1-IRES-PuroR saRNA; polyA encoded on template |