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pKG04056-VEE-eGFP-IRES-BleoR-polyA
(Plasmid #246034)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 246034 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pKG03372-VEE-eGFP-IRES-PuroR-polyA
  • Backbone manufacturer
    Kate Galloway, Addgene plasmid #246033
  • Backbone size w/o insert (bp) 11019
  • Total vector size (bp) 11394
  • Vector type
    Mammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon
  • Selectable markers
    BleoR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Grow at 30 C to minimize truncation of encoded polyA sequence.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BleoR
  • Insert Size (bp)
    375

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pIVT-Flp-2A-BleoR (Kate Galloway, Addgene plasmid #232752)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.24.684179 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKG04056-VEE-eGFP-IRES-BleoR-polyA was a gift from Kate Galloway (Addgene plasmid # 246034 ; http://n2t.net/addgene:246034 ; RRID:Addgene_246034)

  • For your References section:

    Self-amplifying RNA enables rapid, durable, integration-free programming of hiPSCs. Della Santina CM, Ploessl DS, Lindsay-Mosher N, Brown CG, Ehmann ME, Blanch-Asensio A, Chang Kim E, Boyden ES, Galloway KE, Boyer LA. Cell Rep Methods. 2026 Sep 16:101602. doi: 10.1016/j.crmeth.2026.101602. 10.1016/j.crmeth.2026.101602 PubMed 42748925
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