pKG04063-pSHIP-CAG.d-eGFP-IRES-PuroR-bGH
(Plasmid
#246040)
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PurposeMammalian expression vector for eGFP-IRES-PuroR transgene
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Depositing Lab
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Depositing OrganizationMassachusetts Institute of Technology
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepENTR4
- Backbone size w/o insert (bp) 4002
- Total vector size (bp) 5958
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeGFP-IRES-PuroR
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Insert Size (bp)1956
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byT7-VEE-GFP (Steven Dowdy, Addgene plasmid #58977)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.10.24.684179 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKG04063-pSHIP-CAG.d-eGFP-IRES-PuroR-bGH was a gift from Kate Galloway (Addgene plasmid # 246040 ; http://n2t.net/addgene:246040 ; RRID:Addgene_246040) -
For your References section:
Self-amplifying RNA enables rapid, durable, integration-free programming of hiPSCs. Della Santina CM, Ploessl DS, Lindsay-Mosher N, Brown CG, Ehmann ME, Blanch-Asensio A, Chang Kim E, Boyden ES, Galloway KE, Boyer LA. Cell Rep Methods. 2026 Sep 16:101602. doi: 10.1016/j.crmeth.2026.101602. 10.1016/j.crmeth.2026.101602 PubMed 42748925