We narrowed to 15,991 results for: LEA

1. pExp-sfGFP

   Plasmid

   #112573

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8xHis, Strep II, and sfGFP

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-BG4-NLS-mVen

   Plasmid

   #211449

   Purpose

   mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence

   Depositor

   Helmut Pospiech

   Insert

   Anti-DNA G-quadruplex single chain antibody BG4
   

   Tags

   NLS-mVenus

   Expression

   Mammalian

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15-MHL

   Plasmid

   #26092

   Purpose

   SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Sept. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pExp-MsyB

   Plasmid

   #112570

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8His and Strep tag

   Expression

   Bacterial

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-CB-FLPe-P2A-tdTomato

   Plasmid

   #72259

   Purpose

   Express FLPe and tdTomato under CB promoter

   Depositor

   Kazuhiro Oka

   Insert

   FLPe-P2A-tdTomato
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   T230I in tdTomato (please see depositor's co…

   Promoter

   CB promoter and chicken beta-actin + CMV enhancer

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pExp-mCherry

   Plasmid

   #112569

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8xHis, Strep II, and mCherry

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEos4b-Nup50-N-10

   Plasmid

   #57516

   Purpose

   Localization: Nuclear Pore, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Loren Looger, Michael Davidson

   Insert

   Nup50 (NUP50 Human)
   

   Tags

   mEos4b

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pExp-Bla

   Plasmid

   #112561

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8xHis-Bla and Strep tag

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pExp-IF2

   Plasmid

   #112567

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8xHis-IF2 and Strep tag

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. OTUD5

    Plasmid

    #28270

    Depositor

    Cheryl Arrowsmith

    Insert

    OTUD5 (OTUD5 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    OTU DOMAIN (residues 172-339).

    Available Since

    Feb. 10, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

11. mTurquoise-Nesprin3-C-18

    Plasmid

    #55572

    Purpose

    Localization: Nuclear Membrane , Excitation: 434, Emission: 474

    Depositor

    Michael Davidson

    Insert

    Nesprin3 (SYNE3 Human)
    

    Tags

    mTurquoise

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pEYFP-C1-NuMA

    Plasmid

    #28238

    Purpose

    Expresses human NuMA with N-terminal EYFP tag in mammalian cells

    Depositor

    Michael Mancini

    Insert

    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOpen-BstpolLF

    Plasmid

    #165553

    Purpose

    Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    Bst DNA Polymerase, Large Fragment
    

    Use

    Synthetic Biology

    Available Since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pAS1-rab3P::his-24::mNeptune

    Plasmid

    #124350

    Purpose

    Nuclear Localized Pan-Neuronal mNeptune

    Depositor

    Andrew Leifer

    Insert

    rab-3, mNeptune (rab-3 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEos3.2-Nup50-N-10

    Plasmid

    #57479

    Purpose

    Localization: Nuclear Pore, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson

    Insert

    Nup50 (NUP50 Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. C39m

    Plasmid

    #120972

    Purpose

    MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    ccdB toxin
    

    Use

    Synthetic Biology

    Mutation

    BsaI site removed

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTH385-GST-TEV-SUP35

    Plasmid

    #29744

    Depositor

    Tobias von der Haar

    Insert

    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    wild type

    Available Since

    Aug. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pExp-PpiB

    Plasmid

    #112572

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8xHis-PpiB and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. BII-gR-PnTW

    Plasmid

    #133356

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS

    Depositor

    Blair Madison

    Insert

    tdTomato-NLS
    

    Tags

    HA-tagged tdTomato

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAS1-rab-3P::his-24::CyOFP

    Plasmid

    #124347

    Purpose

    Nuclear Localized Pan-Neuronal CyOFP

    Depositor

    Andrew Leifer

    Insert

    rab-3, CyOFP (rab-3 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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