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We narrowed to 9,083 results for: sgRNA

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Showing: 8721 - 8740 of 9083 results

  1. Adeno EA

    Plasmid
    #64071
    Purpose
    Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4
    Depositor
    Andrea Ventura
    Article
    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi
    Insert
    U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
    Use
    Adenoviral
    Tags
    FLAG-Cas9
    Promoter
    U6 and CBh
    Available Since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. pS-sg:GFP

    Plasmid
    #196296
    Purpose
    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
    Use
    CRISPR
    Expression
    Plant
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    196296-plasmid-map-sequence-id-389821

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  3. pCAT077

    Plasmid
    #180511
    Purpose
    Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180511-plasmid-map-sequence-id-359731

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  4. pCAT105

    Plasmid
    #180510
    Purpose
    Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    DpbCasX with R3 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180510-plasmid-map-sequence-id-359734

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  5. pCAT526

    Plasmid
    #180513
    Purpose
    Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv1
    PlmCasX-2A-mNeonGreen
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180513-plasmid-map-sequence-id-359728

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  7. pCAT079

    Plasmid
    #180509
    Purpose
    Plasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    DpbCasX
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    June 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    180509-plasmid-map-sequence-id-364076

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  9. pSL21-VEX

    Plasmid
    #158230
    Purpose
    Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)
    Depositor
    Junwei Shi
    Article
    Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d
    Type
    Empty backbone
    Use
    CRISPR and Retroviral
    Expression
    Mammalian
    Promoter
    U6 promoter for crRNA expression and EFS promoter…
    Available Since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158230-plasmid-map-sequence-id-317503

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  11. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Article
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73796-plasmid-map-sequence-id-237125

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  14. PX458-3xHA-en31FnCas9

    Plasmid
    #201949
    Purpose
    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    3xHA-NLS-en31FnCas9-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    G1243T, E1369R, E1603H on FnCas9
    Promoter
    Cbh
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201949-plasmid-map-sequence-id-405303

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  16. dTAG-IRF8

    Plasmid
    #190620
    Purpose
    Lentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradation
    Depositor
    Junwei Shi
    Article
    Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1
    Insert
    IRF8 (IRF8 Human)
    Use
    Lentiviral
    Tags
    FKBP12_G36V
    Expression
    Mammalian
    Mutation
    synonymous mutation for sgRNA resistance for sgIR…
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    190620-plasmid-map-sequence-id-372992

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  17. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #156432
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    none
    Available Since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156432-plasmid-map-sequence-id-310156

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  20. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Article
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Human, Synthetic)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

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Showing: 8721 - 8740 of 9083 results