We narrowed to 59,826 results for: eng

1. AAV pCAG-mRuby3-WPRE

   Plasmid

   #107744

   Purpose

   Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

   Depositor

   Rylan Larsen

   Article

   Zhuang et al Cell Rep. 2021 Oct 12;37(2):109826. doi:

   Insert

   mRuby3
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. MBP-FUS_FL_12E

   Plasmid

   #98652

   Purpose

   Expresses MBP-tagged full length phosphomimetic FUS

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   12 S/T to E

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC94-pmax-dCas9VP160-2A-puro

   Plasmid

   #48226

   Purpose

   dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
   

   Use

   CRISPR

   Tags

   2A-puro, HA Tag, and VP160

   Expression

   Mammalian

   Mutation

   D10A;H840A (catalytically inactive)

   Promoter

   CAGGS

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Flag-mRIPK1-S321A

   Plasmid

   #115339

   Purpose

   Mammalian expression of Flag-mRIPK1-S321A

   Depositor

   Junying Yuan

   Article

   Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

   Insert

   RIPK1 (Ripk1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S321A

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT-sox10-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80062

   Purpose

   Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Sox10 promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. xCas12i

   Plasmid

   #195333

   Purpose

   vector for encoding a human codon-optimized xCas12i driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   human codon-optimized xCas12i
   

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CBh, CMV, hU6

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. H3S10ph biosensor

   Plasmid

   #120809

   Purpose

   FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
   

   Expression

   Mammalian

   Mutation

   Threonine 3, Threonine 6, and Threonine 11 are mu…

   Promoter

   CMV

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR221 UBR5 ∆UBR

   Plasmid

   #81063

   Purpose

   Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)
   

   Use

   Cloning

   Mutation

   W1235L (bp g3704t)

   Promoter

   N/A

   Available Since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nxph4-2A-CreERT2 Targeting Vector

   Plasmid

   #62219

   Purpose

   Targets CreERT2 to the mouse Nxph4 stop codon

   Depositor

   Hongkui Zeng

   Article

   Harris et al Front Neural Circuits. 2014 Jul 10;8:76.

   Insert

   Nxph4-2A-CreERT2 (Nxph4 Mouse, Bacteriophage)
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Promoter

   Mouse Nxph4

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Flag-mRIPK1-S321E

    Plasmid

    #115340

    Purpose

    Mammalian expression of Flag-mRIPK1-S321E

    Depositor

    Junying Yuan

    Article

    Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

    Insert

    RIPK1 (Ripk1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S321E

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR221 UBR5 ∆HECT

    Plasmid

    #81065

    Purpose

    Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)
    

    Use

    Cloning

    Mutation

    C2768A (bp t8302g and g8303c)

    Promoter

    N/A

    Available Since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR221-EF1.Pro

    Plasmid

    #79488

    Purpose

    MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoter

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    EF1 Promoter (EEF1A1 Human, Synthetic)
    

    Use

    Multisite gateway entry clone

    Promoter

    EF1 Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHRIG-AktDN

    Plasmid

    #53597

    Purpose

    lentiviral expression of dominant negative mouse Akt1 and EGFP

    Depositor

    Heng Zhao

    Article

    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Lentiviral

    Tags

    HA and IRES-EGFP

    Expression

    Mammalian

    Mutation

    Dominant negative (K179M)

    Promoter

    CMV

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

14. MBP-FUS_FL_6E

    Plasmid

    #98655

    Purpose

    Expresses MBP-tagged full length phosphomimetic FUS

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    6 S/T to E

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-A3A(N57Q)-BE3

    Plasmid

    #158157

    Purpose

    T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

    Depositor

    Keith Joung

    Article

    Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

    Insert

    6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
    

    Tags

    6xHis tag on N-terminus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV Cbx5-V5

    Plasmid

    #175105

    Purpose

    Lentiviral expression of mouse Cbx5-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Cbx5 (Cbx5 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ai27

    Plasmid

    #34630

    Purpose

    Cre-dependent expression of hChR2(H134R)-tdTomato, for optogenetic excitation

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

    Insert

    Floxed hChR2(H134R)-tdTomato
    

    Use

    Mouse Targeting

    Tags

    tdTomato

    Mutation

    His 134 has been mutated to Arg

    Promoter

    CAG

    Available Since

    Feb. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV Cbx3-V5

    Plasmid

    #175104

    Purpose

    Lentiviral expression of mouse Cbx3-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Cbx3 (Cbx3 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBABE MDER

    Plasmid

    #13494

    Depositor

    Stephen Tapscott

    Article

    Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90

    Insert

    MyoD1-Estrogen Receptor Fusion Protein (Myod1 Mouse, Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    full length MyoD was fused to aa282-595 of Esr1. …

    Available Since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC95-pmax-dCas9VP160-2A-neo

    Plasmid

    #48227

    Purpose

    dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
    

    Use

    CRISPR

    Tags

    2A-neo, HA Tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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