We narrowed to 16,198 results for: grna

1. ipUSEPR-sg-Hs-PHGDH-4

   Plasmid

   #188678

   Purpose

   sgRNA

   Depositor

   Lewis Cantley

   Insert

   sgPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Mutation

   WT

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. ipUSEPR-sg-Hs-PHGDH-3

   Plasmid

   #188677

   Purpose

   sgRNA

   Depositor

   Lewis Cantley

   Insert

   sgPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Mutation

   WT

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. HCP2

   Plasmid

   #166104

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. HCP3

   Plasmid

   #166105

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. HCP7

   Plasmid

   #166109

   Purpose

   This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.

   Depositor

   Matthias Heinemann

   Insert

   Whi5-sg106 (WHI5 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNSU299_APL3

   Plasmid

   #166073

   Purpose

   Plasmid for constituive spCas9 and tet-inducible APL1-targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   APL3 (APL3 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Tet-inducible

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNSU299_PPE1

   Plasmid

   #166083

   Purpose

   Plasmid for constitutive spCas9 and tet-inducible PPE1 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   PPE1 (PPE1 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Tet-inducible

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRARB.1.0-gDNA

   Plasmid

   #132471

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   RARB (RARB Human)
   

   Use

   CRISPR

   Available Since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. UCT1m

   Plasmid

   #121041

   Purpose

   MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   UCT-part guide RNA (Stanley Qi sequence)
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. C114m

    Plasmid

    #121013

    Purpose

    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    dCas9 with no bbsI or BsaI sites
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pML104-KanMx4

    Plasmid

    #83476

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    KanMX4
    

    Expression

    Yeast

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pML104-HygMx4

    Plasmid

    #83475

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    HphMX4
    

    Expression

    Yeast

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG1guide_RUBY_EggCas9

    Plasmid

    #225983

    Purpose

    To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants

    Depositor

    Imran Siddiqi

    Insert

    Guide RNA against AtAGAMOUS1
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    35s promoter to Drive RUBY and DD45p to drive Cas9

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pML104-NatMx3

    Plasmid

    #83477

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    NatMx3
    

    Expression

    Yeast

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCAS9i_TRP

    Plasmid

    #141253

    Purpose

    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1-TRC.mKO2_shHuR CDS

    Plasmid

    #110426

    Purpose

    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAVL1 HuR
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 (RNA Pol III)

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. ipUSEPR-sg-Hs-PCNA

    Plasmid

    #188686

    Purpose

    control sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPCNA (PCNA Human)
    

    Use

    CRISPR and Lentiviral

    Mutation

    WT

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRDB_031

    Plasmid

    #245331

    Purpose

    Cas12a CRISPRko all-in-one positive control; targets CD47, CD63

    Depositor

    John Doench, David Root

    Insert

    Cas12a, sgRNA CD47, CD63 (CD47 Synthetic)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDB_245

    Plasmid

    #245332

    Purpose

    Cas12a CRISPRko positive control guide; targets CD46

    Depositor

    John Doench, David Root

    Insert

    sgRNA CD46 (CD46 Synthetic)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRDA_837

    Plasmid

    #245328

    Purpose

    Cas9 CRISPRko positive control guide; targets CD55

    Depositor

    John Doench, David Root

    Insert

    sgRNA CD55 (CD55 Synthetic)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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