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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,205 results for: LEA

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Showing: 9541 - 9560 of 12205 results

  1. pcDNA3-CBX1mut-Dronpa

    Plasmid
    #138250
    Purpose
    Triple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.
    Depositor
    Jin Zhang
    Publication
    Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:
    Insert
    CBX1mut-Dronpa
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    138250-plasmid-map-sequence-id-307434

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  4. pmSod1-Q42L-N’His

    Plasmid
    #117708
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q42L
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117708-plasmid-map-sequence-id-227535

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  5. pmSod1-Q123A-N’His

    Plasmid
    #117709
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q123A
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117709-plasmid-map-sequence-id-227504

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  6. pmSod1-D83G-N’His

    Plasmid
    #117702
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    D83G
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117702-plasmid-map-sequence-id-227517

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  7. pEPOR1CB0014

    Plasmid
    #117550
    Purpose
    Level 1 Golden Gate Cassette: AsCas12a expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Publication
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + AsCas12a (with stop codon)+ Nos (pICH41421)
    Expression
    Plant
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117550-plasmid-map-sequence-id-228088

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  8. FAM83B (FAM83BA-c004)

    Plasmid
    #98236
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FAM83BA (FAM83B Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    98236-plasmid-map-sequence-id-227301

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  15. p5A1-T

    Plasmid
    #92017
    Purpose
    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Publication
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with 3 TEV cleavage sites
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Mutation
    S219V
    Available since
    Jan. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    92017-plasmid-map-sequence-id-201852

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Showing: 9541 - 9560 of 12205 results